摘要
目的 探讨柯萨奇病毒B3(CVB3)在亚急型克山病发生发展中的作用。方法 采用原位核酸杂交和反转录 聚合酶链反应 (RT PCR)技术对吉林省病区亚急型克山病尸检心肌组织石蜡包埋块进行检测 ,并对所得DNA片段进行核苷酸序列分析。结果 9例吉林省病区亚急型克山病尸检病例中有 7例出现原位核酸杂交结果阳性 ,阳性率为 78% ,对RT PCR所得DNA片段进行核苷酸序列分析发现它与CVB3有心肌毒性毒株 (CVB30 /Se-)的相应片段序列相同。结论 亚急型克山病病人心肌组织中有柯萨奇病毒B3感染 ,柯萨奇病毒B3参与克山病的发病过程。
Objective To probe into the rel ationship between coxsackievirus B 3 and Keshan disease (KD).Methods Nine autopsy specimens of subacute KD patients were col lected from endemic areas of Jilin province,and enterovirus RNA was detected on them by hybridyzation in situ and reverse transcriptase polymerase chain rea ction (RT-PCR). Results Seven of 9 showed positive signal,with a positive rate 78%.Among these positive specimens,3 obtained DNA segment of 547 bp by RT-PCR.S equencing analysis showed that this segment was one of myocardial virulent strai ns of CVB 3 (CVB 30/Se ).Conclusion From all these data,a conclusion can be drawn that t here must be coxsackievirus B 3 infection in the heart muscle of sub-acute KD patients,the virus involves in KD′s pathogenetic process.
出处
《中国药物与临床》
CAS
2003年第4期313-315,共3页
Chinese Remedies & Clinics
基金
国家自然科学基金资助项目 ( 39870 6 6 8)
