摘要
目的 探讨肺癌易感性标记物CYP1A1以及GSTM1基因多态以及吸烟等其他环境暴露因素与肺癌发生的关系。方法 采用病例 对照研究的方法 ,收集原发性肺癌病例 91例以及非肺部疾患的住院病例 (对照 ) 91例 ,所有的研究对象均采静脉血进行DNA抽提 ,并用PCR方法检测CYP1A1以及GSTM1基因多态 ,同时调查研究对象吸烟等其他环境暴露因素。应用logistic回归分析方法进行单因素和多因素的分析。结果 无论是单因素分析还是多因素分析均未显示出CYP1A1和GSTM1基因多态与肺癌发病的关联。多因素分析结果表明 :文化程度的OR为 0 6 3(95 %CI:0 4 5～0 86 ) ,吸烟量的OR为 1 5 6 (95 %CI:1 14～ 2 14 ) ,无抽油烟机的OR为 3 77(95 %CI:1 4 8～ 9 5 6 ) ,食用动物油的OR为 1 6 7(95 %CI:1 2 5～ 2 2 4 ) ,常吃胡萝卜的OR为 0 4 7(95 %CI:0 2 2～ 0 98) ,饮酒的OR为 6 5 8(95 %CI:1 5 3～ 2 8 30 ) ,家族肺癌史的OR为 3 75 (95 %CI:1 6 4～ 8 5 8)。结论 CYP1A1和GSTM1基因多态与肺癌发病无明显的关联 ,吸烟、饮酒、食用动物油、家族肺癌史以及无抽油烟机是肺癌的危险因素 ,而高文化程度和常吃胡萝卜与降低肺癌风险有关。
Objective To study the relationship between polymorphisms of CYP1A1 MspI, GSTM1, and other environmental exposure and lung cancer. Methods A 1:1 Individual matching case control study was conducted including 91 lung cancer cases and 91 controls collected from the three Hospitals in Guangzhou. All subjects were interviewed with a uniform questionnaire and blood samples were collected for CYP1A1 MspI, and GSTM1 polymorphisms testing by PCR. Logistic regression analysis was used in the data analysis. Results No significant relationship was observed between polymorphisms of CYP1A1 MspI, and GSTM1and an increased risk of lung cancer. The odds ratio related in multivariate conditional Logistic regression was 0.63(95% CI 0.45-0.86) for higher level of education, 1.56 (95% CI 1.14-2.14) for number of cigarettes smoked per day, 3.77 (95% CI 1.48-9.56) for no air exhauster in the kitchen, 1.67 (95% CI 1.25-2.24) for consumption of animal oil, 0.47(95% CI 0.22-0.98) for consumption of more carrot, 6.58 (95% CI 1.53-28.30) for alcohol drinking and 3.75 (95% CI 1.64-8.58) for family history of lung cancer. Conclusion Polymorphisms of CYP1A1 MspI, and GSTM1 were not associated with an increased risk of lung cancer. Cigarette smoking, alcohol drinking, animal oil consumption, family history of lung cancer and poor ventilation in the kitchen was risk factors of lung cancer. Higher level education and more carrot consumption were protective factors.
出处
《华南预防医学》
2003年第4期13-16,共4页
South China Journal of Preventive Medicine
