摘要
背景与目的 肺癌是严重危害人类健康的恶性肿瘤之一，其发病与肺癌人群中某些肺癌相关基因的遗传多态性有关。本研究旨在探讨细胞色素P4501A1（CYP1A1）基因多态性和谷胱甘肽硫转移酶M1（GSTM1）基因多态性与内蒙古人群肺癌易感性的关系。方法 用PCR-RFLP技术分析了原发性肺癌组和住院对照组（各163例）的CYP1A1、GSTM1基因的多态性、基因型分布频率和交互作用。结果 CYP1A1突变型和GSTM1基因缺陷型EGSTM1（-）]频率分布分别为36．8％、65．0％（病例组）和19．0％、48．9％（对照组），二者经χ^2检验差异有显著性（χ^2=12．82，P=0．000；χ^2=9．78，P=0．002）。CYP1A1突变型患肺癌的风险显著增加（OR=2．48，95％CI为1．51～4．08）。GSTM1（-）者患肺癌的风险也显著增加（OR=2．03，95％CI为1．30～3．17）。基因突变的协同分析发现CYP1A1突变型／GSTM1（-）在肺癌组和对照组中的分布频率分别为28．8％和8．0％，二者经χ^2检验有显著性差异（χ^2=23．883，P=0．000）。CYP1A1突变型／GSTM1（-）患肺癌的风险显著增加（OR=4．90，95％CI为2．50～9．83）。无论是在肺癌组还是在对照组，CYP1A1突变型／GSTM1（-）和CYP1A1非突变型／GSTM1（-）在性别间分布频率的差异均无显著性（肺癌组χ^2=0．797，P=0．372；对照组χ^2=0．670，P=0．761）。吸烟与肺癌易感性的统计学分析，结果显示吸烟与肺癌易感性有关（χ^2=14．197，P=0．000），吸烟者患肺癌的风险显著增加（OR=2．33，95％CI为1.50～3．62）。CYP1A1突变型与吸烟关系的协同分析发现，携带CYP1A1突变型基因的吸烟者较携带CYP1A1突变型基因不吸烟者易患肺癌（OR=4．44，95％CI为2．40～8．32，χ^2=23．843，P=0．000）。GSTM1（-）与吸烟关系的协同分析中也发现，携带GSTM1（-）的吸烟者患肺癌的风险显著增加（OR=7．32，95％CI为3．39～15．50，χ^2=36．708，P=0．000）。结论 CYP1A1突变型和GSTM1（-）是内蒙古地区肺癌的易患因素，二者对肺癌的发生有协同作用，吸烟与肺癌的易感性也有关，CYP1A1突变型、GSTM1（-）与吸烟在肺癌的发生上也有相互促进作用。
Background and objective Lung cancer is one of malignant tumors to hurt human health. It has been known that the development of lung cancer may be associated with genetic polymorphism of some lung cancer related genes. The aim of this study is to explore the susceptibility to lung cancer in relation to CYP1A1 and GSTM1 genetic polymorphisms in population of Inner Mongolia. Methods CYP1A1 and GSTM1 genetic polymorphisms were determined by PCR-RFLP in 163 lung cancer cases and healthy controls respectively. Results The frequencies of CYP1A1 variation genotype （vt/vt） and GSTM1（-） were 36. 8% and 65.0% in lung cancer cases and 19.0% and 48.9% in healthy controls respectively. Statistical tests showed significant difference in the frequencies between the two groups （χ^2=12.82, P=0.0001; χ^2= 9.78, P=0.002）. The risk of lung cancer with CYP1A1 variation genotype was significantly higher than those of controls （OR= 2.48, 95% CI=1. 51-4. 08）. The individuals who carried with GSTMI-null genotype had a high risk of lung cancer （OR=2.03, 95% CI=1.30 3. 17）. Combined analysis of the polymorphisms showed that percentage of CYP1A1（vt/vt）/GSTM1（- ） in lung cancer and control groups was 28.8% and 8.0% respectively （χ^2 = 23. 883. P=0.0001）. The people who carried with CYP1A1（vt/vt）/GSTM1（-） had a high risk of lung cancer （OR=4.90. 95% CI=2.50 9.83）. PearsonChi Square of sex differential showed there was no significance between the homozygous variation genotype of CYP1A1/GSTM1 （-） and the other genotypes of CYP1A1/GSTM1（-） neither in lung cancer group （χ^2 =0. 797, P=0. 372） nor in control group （χ^2 =0. 670. P=0.761）. Statistical tests showed that susceptibility to lung cancer was related to smoking （χ^2= 14. 197, P =0.000）, the risk of lung cancer in smoking individuals raised remarkably （OR=2. 33. 95% CI= 1. 50 3.62）. There may bea synergeticinteraction betweenCYP1A1 variationgenotype （vt/vt） and smoking on the elevated susceptibility to lung cancer （χ^2= 23.843, P=0.000）, the smokers who carried with CYP1A1 （vt/ vt） had a significantly higher risk of lung cancer （OR=4. 44, 95% CI=2. 40 8. 32, χ^2 = 23. 813. P= 0.000）. So did the GSTM1（-） and smoking on the elevated susceptibility to lung cancer, the significantly higher risk of lung cancer had also been found in those smokers who carried with （ISTM1（-） （OR=7.32, 95%CI=3. 39-15. 50, χ^2= 36. 708,P=0. 000）. Conclusion The polymorphisms of CYP1A1 vt/vt and GSTM1（-） are the risk factors in lung cancer of Inner Mongolia. Smoking is also related to the susceptibility to lung cancer. There may be a synergetic interaction between CYP1A1 （vt/vt） and GSTM1（-） on the ele rated susceptibility of lung cancer. So do the CYP1A1 （vt/vt）, GSTM1（-） and smoking.
出处
《中国肺癌杂志》
CAS
2006年第5期413-417,共5页
Chinese Journal of Lung Cancer
基金
内蒙古教育厅科学研究基金(NJ03128)资助~~
