摘要
目的 研究饮食诱导肥胖抵抗 (DIO R)和肥胖 (DIO)大鼠血中胰岛素、瘦素 (leptin)和神经肽Y(NPY)水平的差别。方法 采用 5 0只健康雄性SD大鼠 ,随机分为基础组和高脂组 ,分别用基础饲料和高脂饲料喂养 13周 ,然后根据体重筛选出DIO R和DIO组 ,观察体重、摄食量和体脂含量的变化 ,放免法测血清胰岛素、leptin和血浆NPY含量。结果 DIO R大鼠体重、摄食量和体脂含量均明显低于DIO大鼠 (P <0 0 5 ) ;血清胰岛素、血浆NPY含量显著低于DIO大鼠 (P <0 0 5 ) ;高脂饲料使大鼠血清leptin水平明显增加 (P <0 0 5 ) ,但DIO R与DIO大鼠间无明显差别 (P >0 0 5 )。结论 高脂饲料能够诱导SD大鼠发生肥胖和肥胖抵抗 ,胰岛素 leptin NPY反馈环的平衡在肥胖抵抗的发生中起重要作用。
To study the blood hormone level between diet-induced obesity-resistant(DIO-R) rats and diet-induced obesity(DIO) rats in blood hormone. Methods Fifty male Sprague-dawley(SD) rats were randomly divided into control group and high-fat group and they were fed with basic diet and high-fat diet respectively for 13 weeks. DIO-R and DIO rats were selected according to their body weight, then the intake of diet and body fat contents, the level of serum insulin, serum leptin and plasma NPY were determined by radioimmunoassay.The results showed that the weight, diet intake and body fat of DIO-R rats were significantly lower than those of DIO rats(P<0.05).The level of serum insulin and plasma NPY of DIO-R rats were lower than those of DIO rats(P<0.05).The level of serum leptin of rats in high-fat diet group was higher than that of the rat in basic diet group, but there was no significant difference between DIO-R and DIO rats(P>0.05).Conclusion High fat diets can induce SD rat to develop obesity and obesity-resistance. The balance of insulin-leptin-NPY feedback may play a partial of role in resisting diet-induced obesity of rats.
出处
《卫生研究》
CAS
CSCD
北大核心
2003年第3期212-214,共3页
Journal of Hygiene Research
基金
国家自然科学基金课题 (No .30 0 70 655)
