摘要
用本室建立的“宣威”人肺腺癌细胞系SLC—89作抗原制备了三株抗人肺癌单克隆抗体细胞株4E_(12),4F_(12)和4H_2。它们在体外已培养了8个多月,多次液氮冻存后复苏,生长良好,并继续分泌抗体,经检测,所分泌的抗体对肺癌有较好的反应性,相应抗原在常规石蜡组织切片中仍较稳定,因此有可能作为临床病理诊断试剂。
Three monoclonal antibodies against human pulmonory carcinoma, 4E_(12), 4F_(12)and 4H_2, were established with Xuan Wei human pulmonary adenocarcinoma cell line SLC-89 antigen. They had lasted for more than 8 months in Vitro. Having been stored in liquid nitrogen, the cells reviver normally and continued to secrete antibodies. The secreted antibodies could respond well to pulmonary carcinoma. Associated antigens in paraffin section were still stable. Therefore they will be valuable diagnostic reagents in histopathology.
出处
《昆明医学院学报》
1992年第2期6-8,共3页
Journal of Kunming Medical College
关键词
腺癌
单克隆抗体
肺肿瘤
Human pulmonary carcinoma
Monoclonal antibody
