摘要
应用 RAPD技术检测大针茅植物 7个群体的遗传多样性及其分布 ,探索生态因子与遗传分化的相互联系。在大针茅 7个群体中 ,利用 16个有效引物共获得 134条谱带 ,其中多态性谱带 12 0条 ,占 89.5 5 % ,说明个体间发生较高的遗传变异。 7个大针茅群体遗传变异大部分存在于群体内 (5 8.6 6 % ) ,只有少部分存在于群体之间 (4 1.34% )。 7个群体的特异性位点与其地理分布范围相关。
Random amplified polymorphic DNA(RAPD) analyses were used to estimate the genetic diversity of different populations of Stipa grandis and their distribution, as well as the ecological factors in relation to the genetic differentiation of S. grandis . Among a total of 134 bands perceived via 16 primers from 7 populations of S.grandis in different regions,120 bands or 89.55% are polymorphic, showing the high genetic variation of individuals. Most of the molecular variation(58.66%) of the 7 S.grandis populations exists within each of the populations, while only 41.34% exists among the populations. The specific loci of the 7 populations of S.grandis are closely related to their geographical distribution.
出处
《草地学报》
CAS
CSCD
2003年第2期170-177,188,共9页
Acta Agrestia Sinica
基金
国家自然基金 ( 3 0 0 60 0 15 )
内蒙古自然基金 ( 2 0 0 0 13 0 3 )资助
关键词
大针茅
RAPD
遗传多样性
生态分化
Stipa grandis
RAPD
Genetic diversity
Ecological differentiation
