摘要
黄河三角洲野大豆(Glycine soja Sieb. et Zucc.)盐渍群体的耐盐性高于附近的正常群体。群体内个体间耐盐能力差别很大。盐渍群体有比最耐盐的栽培大豆(G.max (L.)Merr.)品种耐盐能力高得多的个体,也有对盐相当敏感的植株。同工酶分析表明群体内高水半多态性,但酶谱与抗性没有相关性。盐渍与正常群体间的遗传一致性高达0.96。用改良的随机扩增多态DNA(RAPD)方法,10个引物扩增得出群体内多态位点百分数为68/188=0.36。看来,绝大多数位点与耐盐能力无关。上述资料说明,盐渍条件下野大豆自然群体的高度遗传多样性和发育变通性,可能足对盐胁迫强度随时随地变化的环境的适应。
A higher level of salt tolerance in saline populations of wild soybean ( Glycine soja Sieb. et Zucc.) has been found in the Yellow River Delta. The levels of salt tolerance for individuals within populations vary widely. In the saline populations, there are a few plants with much higher tolerant level than that of the most tolerant cultivars. such as Morgan, and others as saline sensitive plants. Isozyme analysis showed a high level of genetic diversity, however, no relationship was found between isozymatic patterns and salt tolerance. Saline and normal populations shared similar genetic structure with a genetic distance of 0.045. By using a modified procedure of random amplified polymorphic DNA(RAPD), a higher level of DNA diversity was detected in saline populations. The authors propose that the high level of genetic diversity and developmental flexibility is responsi- ble for adaptation of wild soybean to changing saline conditions.
基金
中国科学院重点研究项目
关键词
野生
大豆
耐盐性
同功酶
DNA
遗传分化
Wild soybean
Natural population
Salt tolerance
Molecular marker
Genetic diversity
Developmental versatility
