摘要
胰抑素(pancreastatin,PS)是一种具有抑制胰岛分泌作用的新肽。本研究用ABC免疫染色法,在Bouin液固定的常规石蜡切片上,研究了胰抑素在豚鼠,猪和人胰腺内的定位和分布,并用相邻切片双标记法,观察了它与胰岛素的共存关系。结果发现,在人胰腺胰抑素样免疫反应(PLI)细胞主要分布于胰岛的周边部。在猪和豚鼠,大部分胰岛细胞呈阳性胰抑素样免疫反应。用相邻薄切片免疫染色技术证明,猪和豚鼠的PLI细胞主要是B细胞。在3个种属胰腺外分泌部的导管和腺泡等处,也均见有PLI细胞分布,在豚鼠胰腺尤为多见。本文对胰抑素在3个种属胰腺不同分布方式的意义进行了讨论。
Pancreastatin, a novel peptide isolated from porcine pancreas, is capable of inhibiting the insulin release and the exocrine secretion of the pancreas. In this study, the localization and distribution of pancreastatin in guinea pig, pig and human pancreas were investigated with ABC immunostaining method combined with the glucose oxidase-DAB-nickle developing technique on paraffin sections. The results showed that in human pancreas pancreastatin-like immunoreactive(PLI)cells were mainly dist- ributed in the periphery of the islets. While in guinea pig and pig pancreas, the majority of islets cells showed positive pancreastatin immunostaining. The coexistence of pancreastatin and insulin in the pancreatic islets of guinea pig and pig were also proved with immunostaining of adjacent thin sections. PLI-cells were also found in the epithelium of the ducts and acini of all investigated pancreas. However, in the exocrine portion of human and pig pancreas, PLI-cells were present in amounts smaller than those found in guinea pig pancreas. The significance of the distribution pattern of pancreastatin was discussed.
出处
《解剖学报》
CAS
CSCD
北大核心
1992年第1期58-62,共5页
Acta Anatomica Sinica
关键词
胰抑素
胰腺
免疫组织化学
人
豚鼠
猪
Pancreastatin
Immunohistochemistry
Pancreas
Human
Pig
Guinea Pig
