摘要
为建立一种稳定的体外分离,培养及鉴定自体骨髓间质干细胞的方法,抽取贵州香猪骨髓液3ml,以1×106细胞浓度培养,2 d后得到贴壁生长的细胞,约10 d后进行传代培养,传代周期约为5-7 d。选原代及10次传代后的贴壁细胞向成骨细胞及肌细胞转化。原代及传代培养的贴壁细胞为纺锤形细胞。两种细胞在体外特殊诱导环境下可定向分化为成骨细胞及肌细胞。通过本方法培养得到的细胞经长期传代后仍具有多能转化活性,确系骨髓间质干细胞。
To establish a method of isolating and obtaining porcine bone marrow mesenchymal stem cells (MSCs)in vitro, and to assess the possibility of MSCs as a new cell source for tissue engineering. Three ml bone marrow was extracted from the posterior superior iliac spine of the mini-pig and culturd by 1×106/ml cells. Some cells were attached to the culture dish after the second day. The adherent cells approached confluence 10 days after plating and then every passage was cultured for 5-7 days. The primary culture and 10th passaged adherent cells were induced to differentiate into osteoblast and myogenic cells. The primary culture and the passaged cells were spindle-shaped in appearance. They both might be induced to differentiate into bone marrow mesenchymal cells and osteoblast and myogenic cells under special environment. The method for isolating and culturing porcine MSCs was feasible.
出处
《上海实验动物科学》
2003年第2期67-69,74,96,共5页
Shanghai Laboratory Animal Science
基金
国家自然科学基金资助项目(30070748)
