摘要
目的介绍一种新的建立大鼠废用性骨质疏松模型的方法,为废用性骨质疏松研究提供经济、便利的实验模型。方法采用腿-尾固定方法将大鼠右后肢固定2周和4周,以左后肢作自身对照,通过观察固定后两后肢肌肉、骨骼的大体形态和骨湿重、灰重、骨密度和生物力学、组织学等指标上有无差别来判定固定的效果。结果固定2和4周,右后肢肌肉明显较左后肢萎缩,骨骼短小;固定2周后,右后肢股骨湿重(303±31)mg比对侧平均下降了约30%,P<0.01,t=4.36。灰重(55.2±6.4)mg,比对侧下降了近50%,P<0.01,t=5.00。骨密度(7.23±0.76)×10-2g/cm2,比对侧下降了30%,P<0.01,t=3.20有统计学差异;同时生物力学指标值也均明显地较左后肢低,结果有统计学差异;组织学显示右后肢胫骨近端骨小梁明显地比对侧稀疏,破骨细胞增加等,符合骨质疏松的组织学改变。结论腿尾固定法是一个成功、简便的制造废用性骨质疏松大鼠模型的新方法。
Aim To recommend a new economical convenient animal model of disuse ost eoporosis in rats provided for the research of disuse osteoporosis. Methods Righ t hindlimbs of rats were immobilized by Limb-tail fixation for 2 and 4 weeks, c ompared with auto-control of left hindlimbs. The wet and gray weight ,bone mine ral density (BMD)and biomechanics of femurs from the right hindlimbs were dete cted to judge the effect of the immobilization. Results After 2 or 4-week immob ilization, the muscle of right hindlimb had a more significant sign of atrophy a nd bones were shorter and smaller, compared with that of the left hindlimbs. At the end of 2 weeks, the wet weight of the right hindlimb was (303±31)mg and gra y weight[(5.2±6.4)mg] of the right hindlimbs reduced by 30%and 50%more than t hose of the left hindlimbs respectively(t=4.36 and 5.00,P< 0.01 ).The BMD of fem urs of the right hindlimbs decreased by more 30 %than that of the left hindlimb s. The biomechanical values of the right hindlimbs were lower than those of the left hindlimbs, with a statistical difference. Histologically, the bone trabecul a of tibia was found more sparsely and ostenoclasts were more abundant in the ri ght hindlimbs, compared with those of the left hindlimbsfewer. Conclusion Limb- tail fixation is a successful and convenient new way to make animal model of dis use osteoporosis in rats.
出处
《中国临床康复》
CSCD
2003年第15期2131-2133,T001,共4页
Chinese Journal of Clinical Rehabilitation
