摘要
目的 建立流式细胞术快速鉴定ABO血型亚型的实验方法。方法 用荧光素(FITC)标记多人份人源抗-A、抗-B,与受检RBC上的相应抗原结合,利用流式细胞仪快速、特异地分辨每个细胞(生物颗粒)获得细胞的散点图,以RBC设定“门”,用Cell quest verl 2.2软件分析,对照管作“mark”,得测定管阳性细胞的百分率。结果 A型RBC其抗A-FITC百分率为97.40％±0.62％,抗B-FITC百分率为0.40％±0.15％;B型RBC其抗A-FITC百分率为0.42％±0.14％,抗B-FITC百分率为98.36％±0.55％。阴性细胞的x±s=0.43％±0.13％,阳性界值>0.85％(>x±3s)。11例ABO亚型FCM测定结果与血清学方法确认的结果完全一致。结论 流式细胞术鉴定ABO亚型的方法,较之常规的血清学方法,具有简便、快速、结果客观、重复性好之优点。
Objective To establish a new flow cytometry-based method to identify the subgroups of ABO. Methods Human source anti-A(B)labeled with FITC(anti-A(B)-FITC) were specifically bound to human RBC. The fluorescence dot plot diagrams of each cells were obtained by FCM. After gating RBC, the diagrams were analyzed by using software-cell quest verl 2.2.Results The anti-A-FITC and anti-B-FITC of blood group A RBC were 97.40% ± 0. 62% and 0. 40%±0.15% respectively. The anti-A-FITC and anti-B-FITC of blood group B RBC were 0. 42% ± 0.14% and 98. 36%±0.55%. The percentage of negative RBC was 0.43%±0. 13% and the threshold value was>0. 85%. FCM method showed consistent results with serologic testing for 11 cases of ABO subgroups. Conclusion FCM is more objective, sensitive, quicker and more precise than routine serologic typing method.
出处
《中国输血杂志》
CAS
CSCD
2003年第3期165-167,共3页
Chinese Journal of Blood Transfusion
