摘要
目的研究急性髓系细胞性白血病(AML)与骨髓增生异常综合征(MDS)患者红细胞A、H抗原表达与基因启动子CpG岛甲基化的相关性。方法应用流式细胞技术检测红细胞表面A、H抗原表达,并应用甲基化特异性PCR(MS-PCR)测定ABO基因启动子CpG岛甲基化。结果 AML与MDS组红细胞表面A抗原阳性表达率和A抗原平均荧光强度分别与对照组相比,差异有统计学意义(P<0.05),而红细胞表面H抗原阳性表达率和H抗原平均荧光强度分别与对照组相比,差异无统计学意义(P>0.05)。AML组与MDS组患者红细胞表面A抗原阳性表达率、A抗原平均荧光强度、H抗原阳性表达率和H抗原平均荧光强度分别比较,差异无统计学意义(P>0.05)。25例AML与MDS患者中发生ABO基因启动子CpG岛甲基化为14例(占56.0%);16例A抗原阳性表达率或(和)A抗原平均荧光强度降低患者中,有8例(占50.0%)出现ABO基因启动子CpG岛甲基化(AML 4例,MDS 4例),8例未出现ABO基因启动子CpG岛甲基化(AML 4例,MDS4例);而9例未见A抗原阳性表达率或(和)A抗原平均荧光强度降低患者中,有6例(占66.7%)出现ABO基因启动子CpG岛甲基化(AML3例,MDS 3例)。结论 AML与MDS由于红细胞表面H抗原转变为A抗原的过程阻断,导致A血型抗原表达降低,且部分患者出现ABO基因启动子CpG岛甲基化。
Objective To study on the relationship between changes of A,H antigen in part of AML and MDS patients and ABO gene promoter CpG island methylation.Methods To adopt flow cytometry technique to test A/H antigen expression and use methylation-specific PCR to test whether or not ABO gene promoter CpG island be methylated.Results There was evident significance in A antigen positive expression rate and mean fluorescence intensity of AML /MDS patients compared with normal control(P<0.05),while there was no significance in H antigen positive expression rate and mean fluorescence intensity(P>0.05).14/25(56%) cases of AML/MDS showed ABO gene promoter CpG island methylation.8 cases of 16 who showed that reduction A antigen positive expression rate and(or) mean fluorescence intensity indicated ABO gene promoter CpG island methylation(4 AML,4 MDS),and the other 8 were not methylated(4 AML,4 MDS).Otherwise,6 of 9 who did not decrease A antigen positive expression rate and(or) mean fluorescence intensity turned out methylation(3 AML,3 MDS).Conclusions Blocking of process of H antigen converted into A antigen leads to reduction of A antigen expression with AML/MDS,and parts of the patients show ABO gene promoter CpG island methylation,but whether it is typically related with ABO gene promotor CpG island methylation,we need more samples to explore.
出处
《临床输血与检验》
CAS
2011年第3期205-211,共7页
Journal of Clinical Transfusion and Laboratory Medicine
关键词
恶性血液病
血型
抗原
基因
Hematological malignancies Blood type Antigen Gene
