摘要
运用实时荧光PCR(Real timePCR)技术建立了对食品中单增李氏菌进行检测的快速方法,设计的引物和探针的序列特异性强,省去凝胶电泳的繁琐,降低了污染的可能性.在对26种病原菌进行检测过程中,运用实时荧光PCR法和经典培养法进行比较,结果表明用实时荧光PCR法检测单核细胞增多李斯特氏菌,更快速、敏感、特异性更高.
In this article,we used realtime PCR technique to establish a rapid method for detection of Listeria monocytogenes in food.The designed primers and Taqman could be amplified by showing excellent feathers of LM.Furthermore,this method omitted the trivial stepgel electrophoresis and reduced probability of contamination.During identifying twentysix pathogenic bacteria,we used realtime PCR method which is more rapid,more sensitive and more specific than conventional culture method for detection of Listeria monocytogenes in food.
出处
《辽宁师范大学学报（自然科学版）》
CAS
2003年第1期73-76,共4页
Journal of Liaoning Normal University:Natural Science Edition
