摘要
目的对比研究3、6、9、12个月龄大鼠的骨髓基质细胞(marrowstromalcells,MSCs)成骨与成脂肪能力的变化。方法培养液中分别加入成骨、成脂肪诱导剂,在不同时间行细胞化学染色观察3和12个月龄两组大鼠MSCs的成骨与成脂肪能力的变化;采用RT-PCR法分别检测3、6、9、12个月龄大鼠的MSCs的Ⅰ型胶原mRNA和脂蛋白脂酶mRNA水平的变化。结果成骨诱导1周后,12个月龄大鼠的MSCs对照与诱导组碱性磷酸酶(alkalinephosphatase,ALP)表达阳性率均低于3个月龄大鼠的同组细胞。成骨诱导12d后,3个月龄大鼠组的MSCs先于12个月龄大鼠组形成钙化。成脂肪诱导第2天,12个月龄大鼠组的MSCs先于3个月龄大鼠组MSCs生成脂滴。RT-PCR检测显示Ⅰ型胶原mRNA水平随月龄增长表达降低,且随诱导时间延长降低幅度变大;脂蛋白脂酶的mRNA水平随月龄增长表达增加,随诱导时间延长增加幅度变大。结论大鼠的MSCs随月龄增长成骨能力降低,成脂肪能力增强。
Objective To measure the osteogenesis and adipogenesis potentiality of rat marrow stromal cells(MSCs)derived from3,6,9,12-month-old doner rats.Methods Rat MSCs were induced to osteoblast or adipocyte by osteogenic inducer or adipogenic inducer.In different times,3and12-month-old rat MSCs were observed by histochemistry staining;the mRNA level of typeⅠcollagen and lipoprotein lipase of3,6,9,12-month-old rat MSCs were measured by RT-PCR.Results The expression of alkaline phosphatase(ALP)of the control group and induced group of12-month old rat MSCs was less than that of3-month old rat MSCs after1-week osteogenic induction.12days later,calcification was observed in3-month old group.Lipid droplets occurred in the cells of12-month old group after2-day adipogenic induction,while these droplets occurred after3-day or4-day induction in3-month old group.The mRNA level of typeⅠcollagen decreased with the increase of age.The mRNA level of lipoprotein lipase of younger rats was lower than that of older rats.Both of the changes were more significantly with the increase of the induction time.Conclusions With the increase of age,the ability of osteogenesis of rat MSCs decreased,but the ability of adipogenesis increased.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
2003年第3期244-249,T001,T002,共8页
Acta Academiae Medicinae Sinicae
基金
国家高技术研究发展计划项目(863项目)(2002AA326080)资助~~
关键词
大鼠骨髓基质细胞
月龄
成骨分化
成脂肪分化
rat marrow stromal cells
age
osteogenic differentiation
adipogenic differentiation
