摘要
目的 探讨肿瘤多肽致敏的白介素 12 (IL 12 )基因修饰的树突状细胞 (DC)对自发性肺转移癌的治疗作用。方法 小鼠足垫注射 3LLLewis肺癌细胞建立自发性肺转移癌模型 ,经骨髓来源的IL 12基因修饰、3LL特异抗原多肽Mut1体外致敏DC(DC IL 12 Mut1)皮下免疫 2次 ,观察荷瘤鼠肺脏质量、肺表面转移结节、存活期的变化及相应免疫指标等变化 ,实验分8组 ,组间差异行t检验 ,生存期行时序检验。结果 与对照病毒组 (DC LacZ Mut1)及未处理DC组相比 ,DC IL 12 Mut1组肺脏质量最轻 (P <0 .0 1)、肺表面转移结节最少 (P <0 .0 1)、存活期最长 (P <0 .0 1) ,其诱导CTL活性 (P <0 .0 1)和NK活性 (P <0 .0 1)最显著。结论 MHCⅠ类限制性肿瘤抗原多肽致敏的IL
Objective To investigate the treatment of spontaneous metastatic lung cancer by tumor antigen-pulsed, interleukin-12(IL-12) gene-modified dendritic cells (DC). Methods The spontaneous metastatic lung cancer model, prepared by injection of the 3LL Lewis lung cancer cells into the footpads of C57BL/6 mice, were treated by subcutaneous vaccination with tumor antigen peptide Mut1-pulsed, IL-12 gene-modified dendritic cells (DC-IL-12/Mut1)derived from the normal bone morrow . After treatment, the lung weight, the number of lung metastatic nodes, the survival time of the tumor-bearing mice were observed, and the NK and CTL activity were determined respectively. The mice were divided into 8 groups with 12 mice in each group. Results Compared with mice treated with Mut1-pulsed, control LacZ gene modified DC and untreated DC, tumor-bearing mice treated with DC-IL-12/Mut1 have the lightest lung weight(P<0.01), the least lung metastatic node number(P<0.01),the longest survival time(P<0.01),also with the induction of potent CTL activity(P<0.01) and NK activity(P<0.01). Conclusion Tumor antigen-pulsed, IL-12 gene-modified dendritic cells have significant therapeutic effects on spontaneous metastatic lung cancer.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2003年第4期299-302,共4页
Immunological Journal
