摘要
本文对11颗年轻人前磨牙牙髓进行了细胞培养。培养后3小时血细胞开始游出,3天后成纤维细胞样细胞开始从组织块边缘长出,平均28天单层细胞形成并按1∶3传代。本实验共培养20代细胞,观察了这些细胞的增殖及硷性磷酸酶活性水平。培养的牙髓细胞维持了体内牙髓的生物学特性,可以用来进行牙髓生物学、药理学研究。
Human dental pulp cells were cultured.Pulp tissues came from normal premolars ofyoung persons.Isolated pulp tissues(2mm of the apical ends were excised)were culturedin Dulbecoo's modified eagle medium(DMEM,Gibco)containing penicillin(100u nits perml),streptomycin(100μg per ml)and 2O% fetal bovine serum at 37℃ under 5% CO_2 and95% air.The medium was changed every 4 days.3 hours after culture,blood cells beganto migrate into the medium;3 days later,fibroblast-like cells grew out from the edge ofpulp tissues;average 28 days later,monolayer cells formed and were subcultured 1:3.Dur-ing the experiment,the 2nd to 20th generation cells were cultured,growth rate and Alka-line-Phosphatase(ALPase)activity of these cells were observed.We found these cells grewstably in higher growth rate and kept higher activity of ALPase(131.27nmol)which was8 times than primary fibroblast(gum)and equal to pulp tissues(in vivo).Although pulpcells cultured were fibroblast-like cells,they were different from primary fibroblasts andkept the biological characteristics of dental pulp tissues(in vivo),can be used to studybiology and pharmacology of dental pulp.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
1990年第2期75-77,共3页
West China Journal of Stomatology
