摘要
通过利用二次旋转组合设计的方法探讨常山胡柚应中黄酮类化合物的最佳提取工艺参数,同时利用化学发光法测定这些黄酮类化合物的抗氧化性。结果表明:提取时间、料:液比、提取温度和乙醇浓度对提取效果都有显著影响,影响最大的因素是提取时间,随着提取时间的增加,提取效果的总的变化呈上升趋势。胡柚皮中黄酮类化合物最佳提取条件为乙醇浓度90%,料:液比1:5,提取时间115min,提取温度20℃,最大提取量为1.98g/L;推荐的工业提取条件以乙醇浓度80% ,料:液比为1:8,提取温度50℃,提取时间100min为宜。另外,实验证明胡柚皮中黄酮类化合物作为自由基清除剂存在构-效关系,是一种良好的自由基清除剂。
The optimum extracting technology of flavonoids in Huyou peel was investigated by using quadratic rotational combination designs. At the same time the antioxidation of these flavonoids was determined by using chemical luminescence method. The results showed that the effect of extraction was affected by time, ratio of material to solvent, temperature and alcohol contents significantly. Among them extracting time was the primary condition. The best extracting conditions were 90% alcohol content, the ratio of material to solvent 1:5 , 115 minutes and 20℃. The maximal extraction rate of the flavonoids was above 1.98 g/L The industrial extracting conditions being recommended are 80% alcohol content, the ratio of material to solvent 1:8, 100 minutes and 50℃ Furthermore, the flavonoids of Huyou peel were proved that they are a value clear reagent of free radical and there is a relationship of structure-effect.
出处
《果树学报》
CAS
CSCD
北大核心
2003年第4期261-265,共5页
Journal of Fruit Science
