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分子生物学技术在马铃薯病毒检测中的应用 被引量:13

Detection of Potato Viruses by Using Molecular Techniques
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摘要 直接应用于马铃薯病毒检测的分子生物学技术主要有 :RT PCR检测技术、指示分子 NASBA检测技术、核酸杂交检测技术三大类 ,以RT PCR技术应用最为广泛。另外病毒外壳蛋白基因的克隆与表达、DNA介导的抗体制备及病毒单链抗体片段筛选等技术在马铃薯病毒免疫学检测技术中也有成功的应用 ,并解决了传统免疫学方法难以解决的抗原制备问题 ,使后者更具应用前景。
出处 《中国马铃薯》 2003年第3期175-179,共5页 Chinese Potato Journal
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参考文献31

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