摘要
目的 研究重组人生长激素 (rhGH)对人胃癌细胞生长的影响。方法 利用体外细胞培养测定不同浓度的rhGH对人胃癌细胞株BGC82 3的生长曲线、细胞抑制率和细胞倍增时间的影响。结果 rhGH在体外无明显促进BGC82 3细胞分裂增殖 ,rhGH组与对照组、rhGH +L OHP组与L OHP组比较差异均无显著意义 (P >0 0 5 ) ,且生长曲线没有升高 ;rhGH +L OHP组与对照组比较细胞抑制率为 6 3 2 %和 5 0 8% (P <0 0 1) ,细胞倍增时间为 82 5h和 39 6h(P <0 0 1) ;或rhGH+L OHP组与对应的rhGH组配对比较 ,细胞抑制率为 6 3 2 %和 1 7% (P <0 0 1) ,细胞倍增时间为82 5h和 37 2h(P <0 0 1)。结论 rhGH在体外无明显促进胃癌细胞的分裂增殖 ,与抗肿瘤药合用可提高抗肿瘤药的疗效。
ObjectiveTo study the effects of recombinant human growth hormone(rhGH) on the growth of human gastric cancer cell.MethodsThe study included control?rhGH?Oxaliplatin(L-OHP) and rhGH+L-OHP treatment groups. Cell inhibition rate and cells doubled time of human gastric cancer line BGC823 were studied on different concentration of rhGH.Results rhGH did not have effect on the multiplication of BGC823 cell line, there was no statistical difference on the cell proliferation between rhGH group and control group or between rhGH+L-OHP group and L-OHP group (P>0.05).Compared with control group, cell inhibition rate significantly increased in rhGH+L-OHP group (63.2% vs. 50.8%,P<0.01). Cells doubled time distinctly lengthened (82.5 h vs. 39.6 h, P<0.01). ConclusionIn vitro rhGH does not accelerate the multiplication of human gastric cancer cells and may increase the curative effects of anticancer drugs on cancer cells.
出处
《中华普通外科杂志》
CSCD
北大核心
2003年第6期361-363,共3页
Chinese Journal of General Surgery
