摘要
对黑曲霉 (AspergillusnigerND3 13 )液态发酵生产植酸酶的发酵过程和发酵动力学进行了研究。在摇瓶试验得到基本发酵工艺参数的基础上 ,应用 10L生物反应器进行放大试验取得了较好的结果 ,其最大菌体量 (干重 )达到 1 19g·10 0mL-1,酶活力达到 3 6u·mL-1。试验通过分批补料手段控制比生长速率的变化 ,得到了描述发酵过程的动力学模型并回归了模型参数。利用数学计算软件对试验数据与模型进行拟合 。
Based on the primary fermentation parameters obtained from flake cultures, the law of growth and metabolism of Asp.niger ND313 and the fermentation kinetic models were studied in an auto control bioreactor. The fermentation in bioreactor was successfully performed with a maximal biomass concentration (dry mass) of 1\^19?g\5 100?mL -1 and a maximal enzyme activity of 3\^6?μ\5mL -1. Then the kinetic formula of cell growth rate, phytase production rate and glucose consumption rate were inferred by controling the specific biomass growth rate. The results of mathematic fitting between model calculated values and experimental values proved the validity and rationality of the kinetic models.
出处
《中国农业大学学报》
CAS
CSCD
北大核心
2003年第2期45-48,60,共5页
Journal of China Agricultural University
