摘要
目的研究胃良恶性病变组织中白细胞介素 8(IL 8)mRNA和血小板反应素 1 (TSP 1 )mRNA表达及其临床病理意义。方法将 5 2例胃癌和 32例胃良性病变标本经 1 0 %福尔马林固定后常规制作石蜡包埋切片 ,采用原位杂交染色法研究其IL 8mRNA和TSP 1mRNA的表达。结果胃癌IL 8mRNA表达阳性率明显高于胃良性病变 (6 1 .5 %vs 6 .2 % ,P <0 .0 1 ) ,TSP 1mRNA表达阳性率明显低于胃良性病变 (5 0 .0 %vs 90 .6 % ,P <0 .0 1 ) ,胃腺癌IL 8mRNA阳性率较明显低于印戒细胞癌 ,而TSP 1mRNA则相反 ,但差异均无显著性(均P >0 .0 5 )。组织学分级Ⅰ级、癌细胞未浸润深肌层及无淋巴结转移病例IL 8mRNA阳性率明显低于组织学分级Ⅲ级、癌细胞浸润深肌层或浆膜层和淋巴结转移病例 ,且两两比较差异均有显著性 (P <0 .0 5 ) ,但TSP 1mRNA表达则相反。结论IL 8mRNA和TSP 1mRNA可能是反映胃癌生物学行为、转移发生和预后的重要生物学标记物 。
ObjectivesTo study on the expressions of IL 8 mRNA, TSP 1 mRNA and their clinicopathological significance in the benign and malignant lesions of stomach.MethodsThe expressions of IL 8 mRNA and TSP 1mRNA were assayed by in situ hybridization technique on the 10% formalin fixed and routinely paraffin embedded sections of specimens taken from 52 cases with gastric cancer and 32 cases with benign lesions of stomach.ResultsThe positive expression rate of IL 8 mRNA in gastric cancers was significantly higher than that of benign stomach lesions (61.5 % vs 6.2 %, P<0.01), but that of TSP 1 mRNA was opposite (50.0 % vs 90.6 %,P<0.01). The positive expression rate of IL 8 mRNA in gastric adenocarcinoma was moderately higher than that of ring cell carcinomas, while that of TSP 1 mRNA in the former was just reversed ; anyhow these differences revealed no statistical significance (P>0.05). The positive expression rate of IL 8 mRNA in patients showing histological grade Ⅰ on specimens, without infiltration into deep muscular layer and without lymph node metastasis was distinctly lower than that in patients classified as histological grade Ⅱ, with infiltration into deep muscular or serous layer and with lymph node metastasis (P<0.05). But the positive expression rates of TSP 1 mRNA in these two types of patients were contrary to those of IL 8 mRNA.There existed a closely negative correlation between the positive expression rate of both mRNAs in tissues of gastric cancer.ConclusionThe IL 8 mRNA and TSP 1 mRNA may be the important biological markers for reflecting biological behaviors, metastasis and prognosis of gastric cancer. Detection of them in benign lesions of stomach has important clinical significance in prevention and early diagnosis of gastric cancer.
出处
《医学临床研究》
CAS
2003年第6期407-410,共4页
Journal of Clinical Research
