摘要
目的 探讨端粒酶活性原位检测在卵巢良、恶性上皮肿瘤诊断与鉴别诊断中的价值。方法 采用端粒酶活性原位检测法 (ISLT法 )和核仁组成区相关蛋白 (AgNORs)技术检测 88例卵巢上皮性肿瘤组织 ,其中囊腺癌 2 6例 ,交界性囊腺瘤 2 1例 ,囊腺瘤 15例 ,囊腺癌癌旁组织 2 6例。结果 卵巢囊腺癌 ,交界性囊腺癌 ,囊腺瘤 ,囊腺癌癌旁组织端粒酶检出率分别为 92 .3% ,4 2 .8% ,0。囊腺癌组端粒酶阳性率与交界性囊腺瘤 ,囊腺瘤 ,囊腺癌癌旁组织存在显著差异 (P <0 .0 1)。其端粒酶阳性检出率与患者年龄 ,肿瘤大小 ,病理类型 ,有无腹水、淋巴结转移 ,分化程度及临床分期无明显相关性 (P >0 .0 5 )。卵巢囊腺癌AgNORs总面积为 0 .92 6 9± 0 .2 0 39μm2 ,与其他组有显著差异 (P <0 .0 1) ,并端粒酶活性强度之间具有显著相关性 (rs=0 .910 ,P <0 .0 1)。交界性囊腺瘤组 9例端粒酶阳性的AgNORs总面积为0 .74 83± 0 .0 36 3μm2 ,12例端粒性酶阴性的AgNORs总面积为 0 .5 870± 0 .1381μm2 ,两者有显著差异 (P <0 .0 1)。结论 LSLT法和AgNORs在卵巢囊腺癌的确诊和交界性囊腺瘤与囊腺瘤的诊断与鉴别诊断中有重要的参考价值。
objective To study the significance of the expression of in situ telkmerase Acticity to pathologic diagnosis and differential diagnosis of human ovarian cystadenocarcinoma.Methods Both of in situ telomerase activity labeling(ISLT)and argyrophil staining method for nucleolar organizer regions(AgNORs)were used in 88 samoles of ovarian epithelial tumors.Results The rate of in situ telomerase activity labeling detected in ovarian Cystadenocarcinoma(OCAC)and their surrounding ovarian tissues(SOT),borderline cystadenoma(BCA)and cystadenoma(CA)were 92.3%(24/26),0(0/26),42.8%(9/21),and 0(0/15)respectively;which were significantly higher in groups of OCAC than that in the groups of SOT,BCA and CA( P <0.01). Frequency and levels of thelomerase activity were not associated with patients'age,tumor size,pathologic type,ascites,lymphnode metastasis,differentiation and clinical stage.The total area of AgNORs of OCAC was 0.9269±0.2039μm 2.Statistically,there was significant defference between OCAC and others ( P <0.01).Meanwhile,the total area of AGNORs of OCAC were correlated significantly with the strength of telomerase activity of others,too( r s=0.910 P <0.01).The total area of AgNORs of the positive expression of in situ telomerase activity labeling of BCA was 0.7483±0.063μm 2,and that of the negative expression was 0.5870±0.1381μm 2.There were significant difference between them( P <0.01).Conclusion The detection of LSLT and AgNORs of OCAC may help it to establish the diagosis and differential diagnosis of BCA and CA.\;
出处
《中国实验诊断学》
2003年第3期252-255,共4页
Chinese Journal of Laboratory Diagnosis
基金
广东省医学科研基金资助项目
编号 :A2 0 0 1713
