摘要
目的观察健脾理气方药物血清对肝癌细胞 SMMC-7721端粒酶活性的影响以及诱导肿瘤细胞凋亡的作用,探讨其抗肿瘤治疗的作用机制。方法采用端粒重复序列扩增(TRAP)结合非变性聚丙烯酰胺凝胶电泳银染法,利用健脾理气方中药灌服新西兰兔后制备含药血清,观察健脾理气方药物血清对体外培养的人肝癌细胞株SMMC-7721端粒酶活性的影响,同时利用流式细胞仪检测和荧光显微镜观察对细胞凋亡的影响。结果健脾理气药物血清在 d4开始对肿瘤细胞的端粒酶活性有抑制作用,电泳条带光密度明显降低;并能诱导肿瘤细胞的凋亡,凋亡的比例随作用时间的延长而升高,流式细胞术检测d2,d4,d6的凋亡比例分别为0.81%,5.16%,8.45%。结论对肿瘤细胞端粒酶活性的抑制和诱导肿瘤细胞凋亡是键脾理气中药抗肿瘤治疗作用机制的一部分。
To investigate the anti-cencer mechanism of dianpi Liqi herbs by observing the inhibition of telomerase activity and apoptosis induced in human liver cancer cell SMMC-7721. METHODS We prepared Jianpi Liqi herbs drug serum by lavaging New Zealand rabbits with the herbs.After liver cancer cell was treated with Jianpi Liqi herbs drug serum, Telomeric Repeat Amplification Protocol(TRAP) combined with polyacrlamide and agarose gels electrophresis(PAGE)silver staining were used to detect the change of telomerase activity and the apoptosis was determined by FCM and fluorescence microscopy. RESULTS Drug serum obviously inhibited the telomerase activity of liver cancer cells from the 4th day after drug serum was incubated.The apoptosis rate determined by FCM in 2,4 and 6 days and control were 0.81%,5.16%, 8.45% and 0% respectively.Cell cycle was arrested in G0/GI phase.The proportion of G0/G1 in 2,4 and 6 days were 37.60%,66.63% and 73.61% respectively. Fluorescence microscopy also showed apoptosis 4 days after drug serum was incubated, CONCLUSION Telomerase activity inhibition and induced apoptosis should be one part of anti-cancer mechanisms of Jianpi Liqi herbs.
出处
《世界华人消化杂志》
CAS
2000年第8期879-882,共4页
World Chinese Journal of Digestology
基金
上海市科技发展基金资助课题
No.964019036~~
关键词
肝肿瘤
肝细胞瘤
端粒酶活性
细胞凋亡
Subject headings liver neoplasm
hepatoma
telomerase activity
apoptosis
serology
electrophoresis
polyacrylamide gel
antineoplastic agents
flow cytometry
