摘要
目的 建立芳香酯酶检测方法并作出方法学评价,探讨芳香酯酶的临床应用。方法 采用有机合成方法合成底物4-乙酰氧基-3-甲氧基-ω-硝基苯乙烯,底物经酶水解生成4-羟基-3-甲氧基-ω-硝基苯乙烯,产物在pH8.6Tris-HCI溶液中显红色。检测520nm处吸光度变化以监测产物生成速度,反映酶活性水平。对90例健康体检者和463例病人血清进行了检测并建立了参考值范围。结果 在肝硬化、肝癌、肝炎、胃癌、尿毒症、肾病综合征、肾炎、冠心病(P<0.01)和高血压(P<0.05)时芳香酯酶均显著降低。在肝硬化、肝癌、肝炎检测中发现芳香酯酶与白蛋白(ALB)、胆碱酯酶(CHE)和直接胆红素(D-BIL)相关系数分别为0.872、0.840,-0.495,经t检验相关系数均具有高度统计意义(P<0.01)。结论 本法简便可靠,可采用自动化和手工两种方法,适合不同条件的单位常规应用。为临床肝硬化、肝癌、肝炎、胃癌、尿毒症、肾病综合征、肾炎、冠心病和高血压的诊断、治疗、预后提供重要信息。
Objective To establish a new method for measurement of serum arylesterase activity and investigate its clinical application. Methods 4 -acetoxy -3 -methoxy-io-nitrostyrene was synthesized in order to study the action of arylesterase on thd substrate. The arylesterase activity from the velocities of reaction was determined by the increasing absorbance of product 4 -hydroxy -3 -methoxy -w -nitrostyrene in pH 8.6 Tris-HCl buffer at 520nm, The levels of serum arylesterase activity of 463 patients and 90 healthy persons were tested to establish a reference ranges. Results The activities of arylesterase showed a remarkable decreasing in the patients with certain liver diseases, gastric carcinoma, uremia, neplrotic syndrome, nephritis, coronary heart disease (P<0.05). There was a correlation between arylesterase and Alb, Che, D-Bil, with the -y 0.872, 0.840 and-0.495 respectively. T-test was showed a significant difference (P<0.01). Conclusion This assay, with its simple and reliable, may be used as routine test in laboratory and served as a target for the diagnosis, therapy and prognosis of some related diseases.
出处
《江西医学检验》
2003年第3期151-154,共4页
Jiangxi Journal of Medical Laboratory Sciences
