摘要
以1株从发病鸽中分离到的新城疫病毒(PNDV/99株)和1株从发病鹅中分离到的新城疫病毒(GNDV/00株)为研究对象,用反转录-聚合酶链式反应(RT-PCR)对它们的F基因进行分段扩增、定向克隆到pUC119质粒载体,然后测定它们的核苷酸序列,并推导出相应的氨基酸序列。PNDV/99株和GNDV/00株的F基因全长1662bp,编码553个氨基酸,它们的裂解位点的氨基酸序列为112G-R-Q-G-R-L117,是NDV弱毒株特有的序列结构。序列分析结果表明,它们2株之间的核苷酸同源率高达99.28%,氨基酸同源率则为100%;它们与国内标准毒株F48E9的核苷酸同源率只有87.48%和87.36%,氨基酸的同源率都为91.68%;而它们与弱毒株LaSota的核苷酸同源率则为98.50%和98.74%,氨基酸同源率都为98.73%。
Two Newcastle disease virus strains, PNDV/99, isolated from a pigeon and GNDV/00, isolated from a goose were investigated. Full-length F gene of the two strains were amplified by reverse transcription and Polymerase chain reaction(RT-PCR) and cloned into pUC119. The genes were sequenced and 1662bp were determined, and the cleavage site on the deduced peptide of the two strains were 112G-R-Q-G-R-L117, indicated they are not virulent NDV strains. The sequence comparision showed 99.28% homology in nucleotide and 100% in amino acid with each other. And they showed 87.48% and 87.36% homology in nucleotide and 91.68% in amino acid with F48E9, while compared with La Sota there are 98.50%, 98.74% and 98.73% respectively.
出处
《上海交通大学学报（农业科学版）》
2003年第2期99-105,共7页
Journal of Shanghai Jiaotong University(Agricultural Science)
基金
上海市科技发展基金项目(983213014)
