摘要
采用5种不同苏云金杆菌菌株进行了菌株交叉混合摇瓶发酵试验和一种组合2 3t发酵罐中试,以及两个优势组合在不同氮源培养基上的混合发酵试验。摇瓶试验获得两个优势组合为GC 91+58和94004+94001,其发酵效价分别为6476和6071IU/μl,晶体蛋白含量分别为0 526%和0 577%。发酵效价分别比GC 91纯培养提高50%和40%,晶体蛋白含量分别比GC 91纯培养提高24%和36%。两种组合在以豆饼粉为主要氮源的培养基上发酵水平分别达到5650和5800IU/μl,明显优于以棉籽饼粉为氮源的培养基。GC 91+58组合在2 3t发酵罐中试发酵效价和晶体蛋白含量达到4520IU/μl和0 467%。
The binary mixed fermentation was studied by shake flasks. Two best combinations from 5 different Bacillus thuringiensis strains were obtained from shake flasks. The potency and crystal protein content of combination of GC91+58 were 6476IU/μl and 0.526%. The potency and crystal protein content of combination of 94001+94004 were 6071IU/μl and 0.577%. Comparing with pure fermentation of GC91, the value of potency increases respectively by 50% and 40%, the value of crystal protein content increases respectively by 24%, 36%. Using new nitrogen source of defatted soybean cake, the potency of the two best combinations were: 5650IU/μl of GC91+58 and 5800IU/μl of 94001+94004. They are much better than using nitrogen source of defatted cotton seed cake. The potency and crystal protein content of GC91+58 in 2300L fermentor were 4520IU/μl and 0.467%.
出处
《中国生物防治》
CSCD
北大核心
2003年第2期66-69,共4页
Chinese Journal of Biological Control
基金
国家"九五"攻关项目(96 005 01 09)
