摘要
Objective:To evaluate the diagnostic potential of cerebrospmal fluid (CSF) levels of β-amyloid protein (Aβ) as biochemical marker for senile dementia in clinical practice. Methods : Sensitive enzyme-linked immunosorbent assay (ELISA) was performed in our lalxrratory to delect the CSF levels of Aβt-40, Aβ1-42 in 54 patients with Alzlteimer's disease (AD), and 30 normal controls (NC). Results: The cut off value of Aβ ratio and Aβ1-42 concentration in NC group provided 54. 51%, 90. 00% sensitivity and 81. 25%, 84. 38% specificity respectively in diagnosis of AD. Conclusion : AD group had a significant decreased level of Aβ1-42 and an increased level of Aβ ratio, compared with NC group.
目的:检测β-淀粉样蛋白(β-amyloid protein,Aβ)两种主要成分Aβ_(1-40)和Aβ_(1-42)在阿尔茨海默病(Alzheier’s Disease,AD)脑脊液中的变化,并探讨其对AD的诊断价值。方法:采用敏感的双抗体夹心酶联免疫吸附法(ELISA),检测54例AD患者脑脊液中Aβ的浓度,并与30例正常对照者(normal controls,NC)脑脊液中的相应成分进行对比。结果:①AD患者脑脊液中Aβ_(1-42)浓度低于正常对照组,而Aβ_(1-40)/Aβ_(1-42)比值高于对照组;②以脑脊液中Aβ比值及Aβ_(1-42)浓度检测诊断AD的敏感性分别为54.51％、90.00％,特异性为84.25％、84.38％。结论:临床上同时检测脑脊液中Aβ_(1-42)浓度及Aβ比值可作为早期诊断AD的辅助指标,具有一定的临床应用价值。
基金
Supported by Grant from National Ministry of Personnel Foundation for Distinguished Young Schotars of China(1998)
