摘要
以溶菌酶、SDS、高氯酸钠等处理提取副结核分枝杆菌C_2株染色体DNA,经限制性内切酶—PSTI消化后,以质粒pBluescript SK为载体,通过T_4DNA连接酶连接,转入DH_5a受体菌,构建了副结核分枝杆菌C_2株的基因文库。快速提取重组质粒,酶切、电泳分析表明,大部分克隆的DNA片段大小在0.5～4kb之间。
Chromosomal DNA of Mycobacterium paratubercuisis C2 which was prepared with Lysozyme, SBS, and Sodium perchlorate, Chromosomal DNA from M paratuberculosis and vector plasmid Bluescript SK were digested with restrictive endonuclease PSTI respectively. The resulting materials were then concentrated with T4 DNA ligase. The Hgatior, maxture was transformed into DH5a. Recotnbinant clones were obtained soon which were digested. The results showed that the insertium sizes of the recombinant clones were 0. 5-4k lobase.
出处
《吉林农业大学学报》
CAS
CSCD
1992年第4期82-84,共3页
Journal of Jilin Agricultural University
