摘要
采用循环电位扫描法在金电极上合成了聚苯胺膜 ,研究了电位扫描范围、扫描速度、溶液pH及苯胺浓度对膜的选择透过性的影响 .在 pH 7.0的磷酸盐缓冲溶液中 ,苯胺起始浓度为 0 .0 5mol/L、电位扫描范围 0～ 0 .9V(vs.SCE)和扫描速度 0 .14V/s条件下得到聚苯胺膜 ,该膜对过氧化氢、抗坏血酸、尿酸的透过率分别为 0 .80 8、0 .0 19、0 .0 12 .将葡萄糖氧化酶固定到聚苯胺膜修饰金电极上得到的葡萄糖氧化酶电极与铂电极构成葡萄糖传感器 .该传感器对葡萄糖的稳态响应时间为 2 5s、线性响应范围为 0 .0 5～2 0 .0 0mmol/L、校正曲线的斜率为 2 .5nA/ (mmol·L-1) ,连续使用 15d响应信号不变 ,使用 4 0d ,葡萄糖的响应电流下降到初始值的 85 % .与无聚苯胺膜的传感器相比 ,稳态响应时间和储存稳定性基本不变 ,校正曲线的斜率减小一半、线性检测下限由 0 .5mmol/L降到 0 .0 5mmol/L ,抗坏血酸的氧化电流仅为原来的7.7% 。
Polyaniline(PANI) was synthesized at Au electrode by potential scan and the effect of the range and sweep rate of potential scan, the pH and the concentration of aniline on the permselectivity of polyaniline were studied. The permeating ratio of H 2O 2, ascorbic acid and uric acid were 0 808, 0 019 and 0 012, respectively, on the membrane prepared for the potential range of 0～0 9 V, sweep rate of 0 14 V/s, pH of 7 0, and 0 05 mol/L of aniline solution. The PANI membrane was used as a permselectivity membrane to construct the glucose oxidase electrode(GOD). The response time, linear range and the slope of the calibration curve of the sensor formed by GOD anode and Pt cathode for glucose detection were 25 s, 2.5 nA/(mmol·L -1 ) and 0.05～20.00 mmol/L respectively.In comparison with the characters of the glucose sensor without the PANI membrane, the response time and the life time were nearly the same, the lower glucose detection limit is lowered from 0.5 mmol/L to 0.05 mml/L and the slope of the calibration curve was halfed. And the response current of uric acid was not detected, the current of ascorbic acid was reduced to 7.7%.
出处
《哈尔滨工业大学学报》
EI
CAS
CSCD
北大核心
2003年第6期691-694,共4页
Journal of Harbin Institute of Technology
基金
哈尔滨工业大学博士后基金资助项目 (GFDQ2 44 0 60 2 9)
