摘要
为了探讨急性白血病患者血浆可溶性L 选择蛋白 (sL selectin)水平和细胞膜结合L 选择蛋白 (L selectin)及其基因表达与急性白血病发病及病情变化的关系及对指导治疗、判断预后的临床意义 ,用酶联免疫吸附双抗体夹心法 (ELISA)、RT PCR及免疫组化法 ,测定 4 0例急性白血病患者的血浆sL selectin水平 ,L selectin基因 (lyam 1)及细胞膜结合L selectin分子的表达。结果显示 :初治及难治性急性白血病患者血浆sL selectin水平较缓解者及对照者均显著增高 (P <0 .0 1) ,而其mRNA及膜结合L selectin表达和L selectin阳性细胞百分率低于完全缓解组(P <0 .0 5 ) ;伴有肝、脾肿大的急性白血病患者较无此体征者血浆sL selectin水平明显增高 (P <0 .0 1) ;且sL se lectin水平与临床过程有关 ;急性白血病患者L selectinmRNA与膜结合L selectin之间显示显著相关性 (γ =0 782 ,P <0 .0 5 )。结论 :急性白血病患者L selectin基因的表达在mRNA水平及蛋白质水平均较对照减少 ,两者有显著相关性 ;监测患者血浆sL
To study the relationship between the level of the soluble L selectin (sL selectin) in plasma and surface L selectin expression on leukemic cells and episode and state of illness in acute leukemia patients, the plasma level of sL selectin was measured by a sandwich enzyme linked immunosorbent assay, and the expressions of surface L selectin and its gene (lyam 1) were detected by immunohistochemistry and RT PCR. The results showed that the levels of sL selectin were significantly higher in untreated and therapy resistant acute leukemia patients, and expression of L selectin mRNA and cell surface L selectinin in untreated and NR patients were significantly lower than that in CR patients and control group (P<0.05). The plasma levels of sL selectin were significantly increased in patients with splenomegaly and hepatomegaly (extramedullary infiltration). The levels of sL selectin were related to the clinical course of the acute leukemia patients. A significant correalation existed between expressions of L selectin mRNA and surface L selectin in acute leukemia patients (γ=0.782, P<0.05). It is concluded that expression of L selectin gene was down regulated in level of mRNA and protein in acute leukemia patients and both changes were highly correlated. Monitoring of the plasma level of sL selectin is possibly useful for early diagnosis of relapse and extramedullary infiltration in acute leukemia.
出处
《中国实验血液学杂志》
CAS
CSCD
2003年第3期251-255,共5页
Journal of Experimental Hematology
