摘要
在液体发酵条件下,以纤维素粉或麦麸为不同碳源及添加碳酸钙和酵母膏对里氏木霉产纤维素酶系不同组分的最高酶活、比酶活及其形成时间、分泌规律等进行了探讨。结果表明:以纤维素粉或麦麸为不同碳源时,各组分的最高酶活、酶活比及其形成时间均有较大差异,并以混合碳源(1 0%纤维素粉+2 0%麦麸)时为最佳,内切型β 1,4 葡聚糖酶(C1)外节型β 1,4 葡聚糖酶(Cx)和β 葡萄糖苷酶(BG)的最高酶活分别达到76 18×10-5,313 25×10-5和135 59×10-5mol·s-1,比酶活分别为72 18×10-5,331 57×10-5和124 29×10-5mol·s-1,最高酶活形成时间为9～10d。添加碳酸钙可明显提高C1和Cx的最高酶活及提高C1的比酶活,降低BG的最高酶活及比酶活和缩短C1和Cx的最高酶活形成时间;添加酵母膏能明显提高BG最高酶活但降低其酶活比;而C1和Cx的比酶活和最高酶活则随其浓度变化而增减不一。3种组分酶的分泌规律和酶系的均衡性极不相同,其中对BG的影响较为滞后。
In the condition of liquid fermentation,we have studied the effects of different factors on highest enzyme activity(ahe),enzyme activity ratio(rea),formation time of highest enzyme activity(FTH)and secretory rule of components in cellulase system in Trichoderma reesei with cellulase,wheat bran and a mixture of two as different carbon sources and additions of CaCO3 and yeast extract paste.The results show that there are great differences among ahe,rea and FTH of different compositions when cellulase and wheat bran are used as carbon sources.The mixed carbon source(10% cellulase+20% wheat bran)results in peak ahe(C1,Cx and BG reach 7618×10-5,31325×10-5,13560×10-5mol·s-1and rhe(C1,Cx and BG reach 7218×10-5,33157×10-5,12429×10-5mol·s-1).The addition of CaCO3 can increase the ahe(C1 and Cx reach 19621×10-5,25490×10-5mol·s-1)and rea of C1(10017×10-5) and Cx(21561×10-5mol·s-1)and reduce the rea of BG(5761×10-5 mol·s-1),formation time of ahe of C1 and Cx distinctly.The addition of yeast extract paste can increase ahe of BG(26061×10-5 mol·s-1)and reduce the rea of BG(389×10-5 mol·s-1),secretory rule dramatically.The equilibrium of all components are very different and their effects on BG are lagged behind.[Ch,3 fig.3 tab.12 ref.]
出处
《浙江林学院学报》
CAS
CSCD
北大核心
2003年第2期146-150,共5页
Journal of Zhejiang Forestry College
基金
国家自然科学基金资助项目(39460060)
关键词
生物化学
里氏木霉
纤维素酶系
产酶特性
均衡性
biochemistry
Trichoderma reesei
cellulase system
characteristics of enzyme production
balance
