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5-氮杂胞苷促进白菜开花的效应分析 被引量：15

Analysis of effects of 5-azacytidine on promoting flowering in non-heading chinese cabbage
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摘要 用5-氮杂胞苷(5-azaC)处理白菜种子,并测定处理后子叶期幼苗的DNA甲基化水平和赤霉素(GA)含量,以分析5-azaC促进白菜开花的效应.结果表明,低浓度5-azaC促进白菜开花,且与低温促进开花的作用具有加成性;但高浓度时却抑制开花,以250μmol/L促进开花的效果最好.5-azaC降低白菜DNA甲基化水平和GA含量,表明5-azaC具有代替低温促进植物开花的作用. The treatment of the seeds with 5azacytidine promoted flowering at low concentration but inhibited flowering at high concentration in nonheading chinese cabbage.The optimum concentration was 250 μmol/L. In addition the effects of 5azacytidine were additive with low temperature. 5azacytidine decreased DNA methylation level and gibberellin content. These results indicated that 5azacytidine could substitute for the effects of low temperature in flower promotion.
作者 李梅兰 曾广文 朱祝军
机构地区 浙江大学园艺系 浙江大学生物科学系
出处 《浙江大学学报（农业与生命科学版）》 CAS CSCD 北大核心 2003年第3期287-290,共4页 Journal of Zhejiang University:Agriculture and Life Sciences
关键词 5-氮杂胞苷 白菜 种子处理 DNA甲基化 赤霉素 开花 促进作用 5-azacytidine non-heading Chinese cabbage flowering DNA methylation low temperature
分类号 S634.3 [农业科学—蔬菜学]
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参考文献9

  • 1Burn J E, Bagnall D J, Metzger J D, et al. DNA methylation, vernalization, and the initiation of flowering[J]. Proc Natl Acad Sci USA, 1993, 90: 287-291.
  • 2Finnegan E J, Genger R K, Kovac K, et al. DNA methylation and the promotion of flowering by vernalization[J]. Proc Natl Acad Sci USA,1998, 95 : 5824-5829.
  • 3LI Shu-xuan,LI Shi—jun(李曙轩,李式军).Flower bud differentiation and head forming of chinese cabbage[J].Acta Horticulturae Sinica(园艺学报),1964,(6):36—40.(in Chinese).
  • 4ZHANG Ming(张明).Molecular marker and genetic analyses of genic male sterile gene in chinese cabbage(白菜核雄性不育基因的分子标记及遗传分析)[D].Hangzhou:Zhejiang University,2000:47.(in Chinese).
  • 5Demeulemeester M A C, Stallen N V, Proft M P D.Degree of DNA methylation in chicory influece of plant age and venalization [J]. Plant science, 1999,142: 101-108.
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  • 7King G J. Morphological development in Brassica oleracea is modulated by in vivo treatment with 5-azacytidine[J] . dour Horticul Sci, 1995, 70(2): 333-342.
  • 8Finnegan E J, Peacock W J, Dennis E S. Reduced DNA methylation in Arabidopsis Thaliana results in abnormal plant development[J]. Proc Natl Acad Sci USA, 1996,93: 8449-8454.
  • 9Blazquez M A, Green R, Nilsson O, et al. Gibberellins promote flowering of Arabidopsis by activating the LEAFY promoter[J]. The Plant Cell, 1998, 10:791-800.

同被引文献254

引证文献15

二级引证文献106

浙江大学学报（农业与生命科学版）
2003年 第3期

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