摘要
目的 观察缺血性脑卒中患者G蛋白 β 3亚单位基因 (GNB3)C82 5T多态性和相关危险因素 ,探讨遗传和环境相互作用在缺血性脑卒中发病机制中的作用。方法 急性缺血性脑卒中患者 72例 ,并按性别、年龄配对设对照组。用聚合酶链反应 (PCR)方法扩增目的基因 ,用限制性内切酶 (BseDI)酶切PCR产物用于基因分型 ,同时观察血压、体重指数、饮酒、膳食、性格等。结果 脑卒中组GNB3C82 5T基因型分布 (基因型频率CC =0 .32 ,CT =0 .5 8,TT=0 .10 )与对照组比较 ,差异有显著性意义 (基因型频率CC =0 .6 5 ,CT =0 .32 ,TT =0 .0 3;χ2 =14 .6 ,P <0 .0 1) ,但在脑卒中患者中有或无原发性高血压亚组之间基因型分布无差异。logistic回归分析显示 ,与脑卒中发病有关联的是收缩压 (SBP)、GNB382 5T等位基因、饮酒和空腹血糖 (FPG)升高。结论 SBP、FPG增高、饮酒和GNB3C82 5T多态性的T等位基因可能是缺血性脑卒中发病的危险因素 ,GNB382 5T等位基因对脑卒中的影响不依赖于血压、饮酒等其他危险因素 ,可能是缺血性脑卒中的独立危险因子。
Objective To explore the relationship between genetic anomaly and risk factors in ischemic stroke and to analyze the polymorphism at position C825T of the gene that encodes the G protein β3 subunit (GNB3) in patients with stroke.Methods Seventy-two patients with ischemic cerebrovascular disease and matched controls were selected. Genotypes of the polymorphisms were determined by polymerase chain reaction (PCR) and the PCR products were digested by restriction endonuclease (BseDI).Life style, dietary, smoking, alcohol consumption, psychological and mental state, body mass index (BMI), etc,were investigated.Results Genotype distribution of the GNB3 C825T genotype was significantly different between patients (CC=0.32, CT=0.58, TT=0.10) and controls (CC=0.65, CT=0.32, TT=0.03;χ2=14.6,P<0.01). Logistic regression analysis showed that the systolic blood pressure(SBP), GNB3 825T allele, alcohol consumption and the elevation of fasting plasma glucose(FPG) were associated with stroke.Conclusion Increased SBP and FPG,alcohol consumption and GNB3 825T allele may be the risk factors for the pathogenesis of ischemic cerebrovascular disease. GNB3 825T allele may take an effect independent of elevated blood pressure and other risk factors.
出处
《中华老年心脑血管病杂志》
CAS
2003年第3期177-180,共4页
Chinese Journal of Geriatric Heart,Brain and Vessel Diseases
关键词
脑血管意外
高血压
危险因素
cerebrovascular accident
hypertension
risk factors
