摘要
目的 了解G蛋白β3 亚基 (GNB3)基因C82 5T多态性与北京地区缺血性脑卒中发病之间的关系。方法 利用PCR和分子杂交技术对北京地区 2 94例缺血性脑卒中患者及 2 80例非脑卒中患者的C82 5T多态性进行检测和分析。结果 C82 5T多态性位点在两组人群中的分布均符合Hardy -weinberg遗传平衡定律 ,缺血性脑卒中患者中CC、CT、TT三种基因型频率分布依次为 30 .2 7%、48.98%、2 0 .75 % ,非脑卒中患者中为 33 .2 1%、47.5 0 %、19.2 9% ,两组人群中 82 5T等位基因频率分别为 0 .45 2和 0 .430 ,均无显著性差异。结论 GNB3基因C82 5T多态性与北京地区缺血性脑卒中发病无关。
Objective To determine the relationship between C825T polymorphism in G protein beta 3 subunit (GNB3) gene and ischemic stroke in Beijing population.Methods 294 patients with cerebral infarction and 280 controls without brain stroke were screened by polymerase chain reaction (PCR) and corresponding C825T polymorphism probe hybridization.Results C825T polymorphism was in agreement with Hardy-Weinberg equilibrium in both groups.The genotype frequencies of CC,CT and TT in case group were 30.27%,48.98%,20.75% respectively,and those in control group were 33.31%,47.50%,and 19.29%. In addition,the 825T allele frequency was 0.452 in patients ,and 0.430 in controls.The two groups had a similar distribution in both genotype and allele frequency.Conclusion C825T polymorphism of GNB3 gene can not be a genetic risk factor of ischemic stroke in Beijin population.
出处
《卒中与神经疾病》
2001年第1期14-16,共3页
Stroke and Nervous Diseases
