摘要
煤焦油样品经提取分离为酸性、碱性和中性组分,后两组分再经硅胶柱制备色谱分作B_1—B_33个级分和N_1—N_66个级分.Ames试验表明,B_2级分致突变活性最高.该级分经HPLC进一步分级表明,其中d、f级分具较高的致突变性经HRGC、HRGC-MS分析,分别从d级分鉴定出苯并喹啉等60多种氮杂多环芳烃,f级分鉴定出氮杂苯并芴等40多种氮杂多环芳烃.
Ames bioassay-directed separation and identification techniques were employed to study low-temperature coal tar from Lurgi coal gasification process. The sample was dissolved in CH2C12 and separated by acid-base extraction into acid, base, and neutral fractions, which were then fractionated into subfractions of A; B1, B2, B2; N1, N2, N3, N4, N5, N6 by using preparative liquid chroma-tography on a silica gel column. The chemical components of those fractions with high mutagenicity were analyzed and identified by high resolution gas ch-romatography(HRGC) and gas chromatography-mass spectrometry(HRGC-MS). The results of Ames test showed that polycyclic aromatic hydrocarbons(N2 neutral fraction) accounted for 62% of mutagenicity of the whole samples, aza-ar-enes(B2 basic fraction)accounted for 9.4% of the total and gave the highest specific mutagenicity of 9.8 Rev/μg. The B2 fraction was further fractionated into 6 subfractions by reverse phase HPLC and 2 subfractions with high activities were analyzed with HRGC and HRGC-MS. Many mutagenic aza-arenes, such as benzacridine, aza-pyrene or aza-fluoranthene, aza-benzofluorene, aza-be-nzopyrene or aza-benzofluranthene, and dibenzacridine, etc., were tentatively identified.
出处
《环境科学学报》
CAS
CSSCI
CSCD
北大核心
1992年第3期306-315,共10页
Acta Scientiae Circumstantiae
基金
国家自然科学基金资助项目
