摘要
目的 建立一种分离血液中肿瘤细胞的磁性激活细胞分选 (MACS)技术并评价其临床价值。方法 采用胃肿瘤细胞膜表位的MG7、CEA、QY和MMP9单抗分别与溴化氰活化的Sepharose 4B磁珠交联 ,从胃癌患者外周血、门静脉血中分离出肿瘤细胞。结果 应用MACS技术从外周血中分离出肿瘤细胞的阳性率为4 9.15 % (2 9/5 9) ,从门静脉血中分离出肿瘤细胞的阳性率为 90 .32 % (2 8/31) ,两者比较差异有显著性 (P <0 .0 5 )。用MACS技术从外周血、门静脉血中分离出肿瘤细胞的阳性率与患者临床分期无关。结论 MACS技术是一种有效分离肿瘤细胞的可靠方法 ,可从胃癌患者外周血中分离出少量肿瘤细胞 ,有助于进一步探讨恶性肿瘤的发生、发展、浸润转移。
Objective To establish a method for the separation of circulating tumor cells in the peripheral blood based on the magnetic actived cell sorting(MACS) technique and evaluate its clinical value. Methods Monoclonal antibodies to the gastrointestinal carcinoma cell membrane epitopes MG7, CEA, QY and MMP9 were conjugated to immunomagnetic beads with CNBr\|actived Sepharose 4B. Using these immunomagnetic beads the carcinoma cells were separated from the peripheral blood and portal venous blood. Results In 29 of 59(49.15%) samples from peripheral blood and 28 of 31(90.32%) samples from portal venous the carcinoma cells were determined using the magnetic cell sorting (MACS). We have found that there was no relationship between the samples of separated tumor cells and TNM stage. Conclusions MACS appears to be an efficient technique to detect disseminated tumor cells. It can separate carcinoma cells from the peripheral blood with a few carcinoma cells in gastrointestial carcinoma patient. It will contribute to studying the etiology, development, metastasis, and multidrug resistance of tumor.
出处
《上海医学检验杂志》
北大核心
2003年第3期145-147,共3页
Shanghai Journal of Medical Laboratory Sciences
基金
浙江省卫生厅科研基金资助项目 ( 4 910 3 0 w10 0 0 5 )
