摘要
目的探讨门静脉血癌细胞与大肠癌肝转移的关系。方法以非核素ＲＴＰＣＲ方法，对５４例大肠癌患者门静脉血中结肠癌细胞的标志物细胞角质蛋白２０（ＣＫ２０）进行了检测。结果５４例中，４１例门静脉血中检出ＣＫ２０阳性表达，阳性率７５９％，ＣＫ２０阳性检出与Ｄｕｋｅｓ分期、肝转移存在显著性相关，随着Ｄｕｋｅｓ分期的进展ＣＫ２０阳性率显著增加。随访发现，门静脉血ＣＫ２０扩增阳性者，术后发生肝转移机会增加（２９１％）。结论检测门静脉血微转移癌细胞可提高大肠癌临床分期的准确性，帮助综合判断患者预后，且可能有助于早期诊断大肠癌肝脏微小转移。
Objective To study the relation between cancer cells in portal vein blood and liver metastasis of colorectal carcinoma. Methods The presence of CK20 mRNA was investigated by non isotope RT PCR in 54 cases. Results The positive rate of CK2O mRNA in portal vein blood was 75 9%. The positive cases were significantly correlated with Dukes stage and live metastasis. There was a higher probability of liver metastasis in positive cases after operation. Conclusions The detection of CK20 in portal vein blood may improve the accuracy of clinical staging and diagnose liver micrometastasis of colorectal carcinoma.
出处
《中华外科杂志》
CAS
CSCD
北大核心
1999年第4期214-215,共2页
Chinese Journal of Surgery
关键词
大肠肿瘤
肝转移
门静脉血
癌细胞
检测
Colorectal neoplasm Neoplasm metastasis Portal vein Keratin Reverse transcriptase polymerase chain reaction
