摘要
目的 探讨外源核酸对老龄大鼠的生理机能和肝、脑形态学影响。方法 选取健康老龄 (2 0月龄 )Wistar雄性大鼠随机分为高、中、低 3个剂量组和老龄对照组、另取青龄 (3月龄 )作为青龄对照组 ,高、中、低剂量组在标准饲料基础上 ,每日每只分别添加酵母RNA93 75mg、4 6 88mg和9 38mg ,饲喂 4周。结果 高剂量组超氧化物歧化酶 (SOD)活性、血清高密度脂蛋白 (HDL)和生长激素水平均高于老龄对照组 ,酵母RNA补充各组丙二醛含量均低于老龄对照组 ,高、中剂量组血清睾酮达到青龄对照组水平 ,而老龄动物各组间雌二醇水平未见统计学差异 ;补充酵母RNA组的老龄大鼠脑神经元数与老龄对照组大鼠相比有增多趋势并存在剂量效应关系 ,但没有统计学差异 ;肝细胞核面积与核浆比均大于老龄对照组 ,其中核浆比具有统计学差异。结论 酵母RNA可能具有改善老龄大鼠生理机能和肝、脑组织形态学改变的作用 ,并可能具有量 效关系。
Objective To study the effect of exogenous nucleic acid on physical functions, morphology of hepatic cells and brain neurons in aged rats. Methods Thirty two aged Wistar rats (20 month-old) were divided randomly into four groups (one aged control group and three aged experimental groups) and eight young rats(3 month-old) was set as young control group. Control groups were fed on standard chow and experimental groups were fed on standard chow supplemented with 93.75 mg/kg (high-dosage group), 46.88 mg/kg (middle-dosage group) and 9.38 mg/kg (low-dosage group) of yeast RNA respectively. SOD, MDA, HDL, sex hormone and growth hormone were determined at the end of a 4-week observation. The microcosmic images of the hepatic cells and brain neurons using the image-pro plus (V.4.0) were also observed. Results SOD, serum HDL and growth hormone levels in the high dosage group were significantly higher ( P <0.05) than that in the aged control group, and the levels were not different from that in the young control group. MDA level of all yeast RNA supplemented groups was significantly lower than that of aged control group ( P <0.05) and that was not different from the young control group. Serum testosterone of the high and middle dosage groups reached the level of young control group, and that was much higher than the aged control and low dosage group ( P <0.05). Estradiol levels among the aged rats were not different, and those were much lower than the young control group ( P <0.05). Much more number of brain neurons were observed in the high-dose group than other aged rats ( P <0.05). Brain neurons, hepatic cells and karyons in the high-dose group were bigger than that in other aged rats ( P <0.05). Conclusion Exogenous yeast RNA might play an important role in physical functions, the morphology of brain neurons and hepatic cells in natural aged rats. There might have a dose-effect relationship in the process.
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
2003年第3期158-160,F004,共4页
Chinese Journal of Preventive Medicine
基金
国家自然科学基金资助 (3 9870 660 )
