摘要
目的 探讨前列腺增生组织中 bcl- 2和 bax的表达以及 β射线内照射对 bcl- 2和 bax表达的影响。方法 应用免疫组织化学法检测 9例正常前列腺 (NP)、1 5例良性前列腺增生 (BPH)及 35例实施 90 Sr/ 90 Yβ射线照射后前列腺增生组织中 bcl- 2和 bax的表达。结果 NP和 BPH上皮组织 bcl- 2阳性表达均高于间质 (P<0 .0 1 ) ;BPH组织上皮和间质中 bcl- 2表达阳性率高于 NP(P<0 .0 1 ) ;NP上皮细胞中 bax表达高于 BPH(P<0 .0 5) ;BPH上皮和间质细胞中 bcl- 2表达高于 bax(P<0 .0 1 ) ;NP上皮和间质中 bcl- 2与 bax表达无显著差异 (P>0 .0 5)。与对照组相比 ,应用 90 Sr/ 90 Yβ射线内照射 4、7、1 5d后 BPH上皮和间质中 bcl- 2明显下降 ,bax阳性细胞率明显升高 (P<0 .0 1 )。结论 bcl- 2和 bax与前列腺细胞凋亡的调节有关 ,β射线能使前列腺组织中 bcl- 2和 bax含量发生变化 ,促使前列腺细胞凋亡。
Objective To investigate the expressions of bcl-2 and bax in benign prostatic hyperplasia (BPH) and the effect of β-rays on bcl-2 and bax.Methods The expressions of bcl-2 and bax were studied by means of immunohistochemical method in 9 normal prostate (NP) and 15 BPH and 35 patients treated with 90Sr/90Y prostatic hyperplasia applicator. Results The expressions of bcl-2 in epithelia of NP and BPH were higher than that in stroma (P<0.01). The expressions of bcl-2 in epithelia and stroma of BPH were higher than that in NP (P<0.01). The expressions of bax in epithelia of NP were higher than that in BPH (P<0.05). However the expressions of bcl-2 in epithelia and stroma of BPH were higher than bax (P<0.01). Compared with the control group, the expressions of bcl-2 in epithelia and stroma of BPH treated with 90Sr/90Y prostatic hyperplasia applicator decreased and the expressions of bax increased (P<0.01).Conclusions bcl-2 gene and bax gene may play an important role in the regulation of prostatic apoptosis and the treatment of β-rays can accelerate the apoptosis of prostatic tissues.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2003年第5期281-282,共2页
Chinese Journal of Gerontology
