摘要
目的 :了解乙型肝炎病毒(HBV)感染者体内HBV—DNA含量 ,并探讨其临床意义。方法 :本文采用荧光定量聚合酶链反应(FQ—PCR)和ELISA两种方法同时检测了822份血清 ,并对结果进行了对比分析 ,结果 :266例HBsAg( +)、HBeAg( +)、HBcAg( +)组的血清HBV—DNA检出率为87 22 %(232例) ,平均拷贝数为1 72×109.ml;211例HBsAg( +)、HBeAb( +)、HBvAb( +)组血清HBV—DNA检出率为21 27 %(47例) ,平均拷贝数为6 66×108/ml,190例HBsAg( +)、HBcAb( +)组血清HBV—DNA检出率为37 37%(71例) ,平均拷贝数为4 56×108/ml;47例HBVm全阴性组血清HBV—DNA检出率为8 51%(4例) ,平均拷贝数为1 54×108/ml。结论 :HBVm阴性的病人也可能有HBV—DNA阳性 ,因此为临床提供HBV感染、复制及传染性的判断以及指导治疗 ,HBV—DNA定量PCR检测具有重要的意义。
Objective∶To study the reationship of HBV-DNA in serum of patients with hepatitis viral viral B.Method∶822 clinical serum sampies were tested by Fluorescence quantitaive PCR assay (FQ-PCR),using ELISA as contrast.Results∶ln266 HBsAg+/HBeAg+/HbcAb+samples,FQ-PCR results were positive,the average level of HBV-DNA was1.72×109/ml with a positive rate of 87.22%.ln 211HBsAg+/HBeAb+/HBcAb+samples the average level was 6 66×108/ml with a positive rate of 21 27%.ln190HBsAg+/HBcAb+ samples the amount was 1 54×108/ml with a positive rate of 37 37%.ln47 HBVm(-)samples,the amount of HBV-DNA was 1 54×108/ml with a posititve rate of 8 51%.Conclusion∶The results showed that HBV-DNA could be positive by FQ-PCR,even it may be negativ for HBVm,So FQ-PCR can be used as another good monitor the state of HBV infection and complivation.
