摘要
为了解传染性非典型肺炎患者早期的免疫状态 ,采用流式细胞仪以三色荧光标记的单克隆抗体 (CD4 FTTC/CD8 PE/CD3 PC5 )检测入院时 30例非典型肺炎或疑似患者的外周血T细胞亚群比例 ,并作绝对计数和计算CD4 +/CD8+比值。结果表明 ,11例非典型肺炎患者的CD3+、CD4 +T淋巴细胞比例、CD4 +/CD8+比值和CD3+、CD4 +和CD8+T淋巴细胞计数均明显低于 34例健康人 (P <0 0 0 1)。如CD4 +T细胞比例从健康人的 4 0 6 %降至 14 5 % ,细胞计数从健康人的 712 1/mm3 降至 15 1 1/mm3。患者CD8+T淋巴细胞比例也有一定程度的下降 (从健康人的 2 8 7%降至 2 2 6 % ,P <0 0 5 )。 19例疑似患者的上述大部分指标亦较健康人群有明显降低 (P <0 0 0 1)。非典型肺炎患者组与疑似患者组比较 ,前者CD3+T细胞和CD4 +T细胞比例进一步下降 (P <0 0 1) ,CD4 +/CD8+比例也倒置至 0 70。患者病程早期CD4 +T细胞比例下降可引起T细胞免疫功能低下 ,表明非典型肺炎和免疫功能失调有关。
To evaluate the immunological status of the patients with severe acute respiratory syndrome (SARS), T cell subsets from 30 blood samples (11 for SARS and 19 for suspects) were determined by analyzing cell surface markers with flow cytometry The proportions of CD3 +, CD4 + and CD8 + T subsets, together with the cell counts and the CD4 +/CD8 + ratio, are significantly depressed in SARS patients when compared with those in 34 healthy subjects ( P <0 001). The CD4 +T subset, for example, could be depressed from 40 6% to 14 5% in terms of proportion and from 712 1 to 151 1 in terms of cell counting (cell/mm 3) A depression also appeared for CD8 + T subset (28 7% to 22 6%, P <0 05) Similar results were observed for the suspected patients in comparison with the control group ( P <0 001) When the CD3 + and CD4 + T lymphocytes were compared between the SARS patients and the suspects, a much lower proportions were detected in the former, especially for CD4 + T cells ( P <0 01) The ratio of the CD4 + to the CD8 + T cells is thus reversed to 0 70 It is obviously that there existed immunological disorders in our SARS patients at the early stage, characterized by decreasing of CD4 + T lymphocytes
出处
《上海免疫学杂志》
CSCD
北大核心
2003年第3期152-154,共3页
Shanghai Journal of Immunology
