摘要
目的 对河北汉族群体 6个短串联重复序列 ( short tandem repeat,STR)基因频率研究 ,以期得到河北汉族群体 D7S82 0、D19S2 5 3、D12 S391、D5 S818、D16 S5 39和 D8S1179基因座的群体遗传学数据。方法 随机抽取 173名河北省汉族人群无血缘关系个体的静脉血 ,应用 PCR技术扩增上述 6个短串联重复序列基因座 ,采用高分辨的聚丙烯酰胺凝胶垂直板电泳 ,银染显色分析。结果 得到了河北汉族这 6个位点的遗传多态分布。D7S82 0、D19S2 5 3、D12 S391、D5 S818、D16 S5 39、D8S1179基因座的杂合度分别为 :0 .82 8、0 .75 7、0 .76 9、0 .837、0 .785、0 .85 2。个体识别率分别为 :0 .914、0 .919、0 .94 0、0 .90 9、0 .917、0 .94 4。非父排除率分别为 :0 .6 18、0 .74 0、0 .80 1、0 .5 5 7、0 .6 5 5、0 .6 96。遗传多态性信息量分别为 :0 .771、0 .76 0、0 .76 2、0 .70 8、0 .776、0 .794。结论 上述 6个 STR基因座基因频率分布与 Hardy- Weinberg平衡吻合良好 ,在河北汉族群体中具有较高的非父排除率与个体识别率 。
Objective: This article reports a population genetic study on six short tandem repeat (STR) loci, D7S820,D19S253,D12S391,DSS818,D16SS39 and D8S1179, in a sample of unrelated Chinese Han individuals (n = 122-173) living in Hebei province. Methods: DNA extraction from blood samples (200 in number) and multiplex amplification of the above six loci were carried out. Using denaturing polyacrylamide gel electrophoresis and silver stain, the authors investigated the distribution of allele frequencies of the six loci in Han population in Hebei province. Results: The STR polymorphisms at all of the six loci were observed in Chinese Han population in Hebei province. The observed heterozygosities of D7S820,D19S253,D12S391,D5S818,D16S539 and D8S1179 were 0.828,0.757,0.769,0.837,0.785 and 0. 852, respectively. The measured values of the power of discrimination (PD) were 0.914,0.919,0.940,0. 909,0.917,0.944; of the mean exculsion chance (MEC) 0.618,0.740,0.801,0.557,0.655,0.696 and of the polymorphism information content (PIC) in Chinese 0.771,0.760,0.762,0.708,0.776 and 0.794, respectively. Conclusion: The genotype distributions of the six STR were in accordance with Hardy-Weinberg equilibrium. The numerical values of the PD and MEC are relatively high in Hebei province, and thus can be of significant application in population genetics and forensic medicine.
出处
《中华医学遗传学杂志》
EI
CAS
CSCD
2003年第3期259-261,共3页
Chinese Journal of Medical Genetics
