摘要
目的研究ＳＴＲ位点Ｄ１９Ｓ２５３和Ｄ８Ｓ１１７９的多态性，为法医学应用提供基础数据。方法应用ＰＣＲ及ＰＡＧ电泳技术对武汉地区２００多名汉族无关个体进行了调查。结果两位点各检出９个等位基因，首次获得汉族人群频率分布。两位点基因型频率分布符合Ｈａｒｄｙ－Ｗｅｉｎｂｅｒｇ平衡。家系调查证实了等位基因的传递遵循孟德尔遗传规律，观察１００次减数分裂未发现突变基因。Ｄ１９Ｓ２５３和Ｄ８Ｓ１１７９位点的杂合度观察值分别为０．８０８９和０．８７１２，多态性信息含量分别为０．７７５４和０．８２５８。两位点联合ＰＤ值为０．９９６６，累积非父排除率为０．８７９１。结论表明这两个多态位点在法医学个人识别及亲子鉴定中是很有价值的遗传标记系统。
Objective This article reports a population genetic study on two short tandem repeat loci,D19S253 and D8S1179,in a sample of unrelated Chinese individuals (n=225-233) living in Wuhan.Methods PCR and PAGE. Results Nine alleles were identified for the two loci,the ranges of fragment size were 209bp-241bp and 174bp-206bp respectively.The genotypes distributions of D19S253 and D8S1179 were in accordance with HardyWeinberg equilibrium.Family studies confirmed Mendelian inheritance of alleles.No mutation was detected in 100 meiosis for the two STR loci studied.The observed heterozygosities of D19S253 and D8S1179 were 0.8089 and 0.8712.The polymorphism information content(PIC) in Chinese was calculated as 07754 for D19S253 and 08258 for D8S1179.The power of discrimination(PD) and mean exclusion chance(MEC) were 09309 and 06133 for D19S253 and 09515 and 06874 for D8S1179 respectively.The combined PD and cumulative chance of exclusion were calculated as 09966 and 0.8791. Conclusion The results demonstrate that the combined use of the two polymorphic systems provides a high power of discrimination and power of exclusion for use in forensic identification and paternity testing.
出处
《中华医学遗传学杂志》
EI
CAS
CSCD
北大核心
1998年第4期228-231,共4页
Chinese Journal of Medical Genetics
