摘要
目的 探讨对氧磷酶 2 ( paraoxonase2 ,PON2 )基因多态性与 2型糖尿病 ( type2 diabetesmellitus,T2 DM)患者合并缺血性脑卒中 ( ischemic stroke,IS)的关系。方法 用聚合酶链反应 -限制性片段长度多态性分析法探查 PON2基因 C311S多态性在 T2 DM合并 IS组、T2 DM无 IS组以及正常对照组的基因频率。结果 发现中国人存在 PON2基因 C311S多态性 ,C/S等位基因频率为 0 .14 5 /0 .85 5。T2 DM合并 IS组患者 PON2基因的 C等位基因频率显著高于 T2 DM无 IS组和正常对照组 ,差异有显著性 ( P<0 .0 5 )。结论 中国人 2型糖尿病患者 PON2基因第 311位密码子的多态性与并发缺血性脑卒中有关 ,C等位基因是
Objective: To investigate the association between the C311S polymorphism of paraoxonase 2 (PON2) gene and ischemic stroke in Chinese type 2 diabetes mellitus (T2DM) patients. Methods: A case-control study of 279 Chinese subjects (including 162 T2DM with or without ischemic stroke and 117 non-diabetic control) was performed. Genotype frequencies of C311S polymorphism were studied by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis with DdeI digestion. Results: C311S polymorphism of PON2 gene was detected in Chinese with the C/S allele frequencies 0. 145 and 0. 855. The frequency distribution showed significant difference between Chinese and Asian Indian. Furthermore, the genotype distribution (SS, CS and CC) of the PON2 C311S gene polymorphism exhibited a significant difference between T2DM patients complicated with ischemic stroke and T2DM without ischemic stroke, the former had a significantly higher C allele frequency (P < 0. 05). Conclusion: The above data indicate that the polymorphism at codon 311 (Cys&rarrSer) in the PON2 gene is associated with ischemic morbidity in Chinese T2DM patients and C allele might be a risk factor.
出处
《中华医学遗传学杂志》
EI
CAS
CSCD
2003年第3期215-219,共5页
Chinese Journal of Medical Genetics
基金
广东省科委重大项目 (99M0 4 81 6G)~~
