摘要
目的 从基因水平了解T淋巴细胞在急性排斥反应中作用的分子机制。方法 建立大鼠肝移植急性排斥反应模型 ,并设对照组 ,利用 4 0 96条大鼠cDNA克隆的基因芯片从来自排斥组和对照组的T淋巴细胞中抽提、纯化mRNA ,逆转录成cDNA ,荧光标记后与芯片杂交 ,扫描后筛选出差异表达的基因。结果 在 4 0 96个基因中共发现差异表达基因 190条 ,其中 10条差异表达明显、已知功能的免疫相关基因为 :主要组织相容性复合物 (3条 )和CD3抗原 (1条 )相关基因 ;干扰素调节因子 1、上皮生长因子受体、转化生长因子相关基因各 1条 ;白细胞蛋白酶抑制因子、急性期蛋白抑制因子 3相关基因各 1条 ;补体因子 1基因 1条。结论 T淋巴细胞在肝移植术后急性排斥反应中的作用机制涉及多个基因 ;基因芯片技术是一种高效。
Objective To investigate the gene expression pattern and molecule mechanism of T lymphocyte during acute rejection after liver transplantation.Methods The mRNA of T lymphocyte from allo transplantation group (Wistar→SD, n =5) with histologic evidence of acute rejection and iso transplantation group (SD→SD, n =5) without histologic evidence of acute rejection was analyzed for the exression of 4 096 rat genes using microarray. Results Comparative analysis revealed that 190 genes out of the 4 096 rat genes had differential expression (>2 fold or < 0.5 fold), in which 10 immunologic genes with obviously differential expression and known function included MHCⅡantigen, CD3 antigen, interferon regulatory factor 1, acute phase protein alpha 1 inhibitor 3, secretory leukocyte protease inhibitor, epidermal growth factor receptor, transforming growth factor beta and complement factor I related gene. Conclusion The effect of T lymphocyte during acute rejection implicated various genes. The microarray technology is a high effective and high stable method.
出处
《中华器官移植杂志》
CAS
CSCD
北大核心
2003年第3期153-156,共4页
Chinese Journal of Organ Transplantation
基金
广东省医学科学技术研究基金资助项目 (A199914 1)
