摘要
为研究严重烧伤对大鼠肠上皮细胞线粒体呼吸功能的影响及其机制 ,采用 30 %体表面积Ⅲ度烧伤大鼠模型 ,观察烧伤后线粒体Ⅲ态呼吸 (ST3)、Ⅳ态呼吸 (ST4 )、呼吸控制率 (RCR)、磷氧比 (P O)、肠道氧摄取率 (Oext)及肠粘膜血流量 (IMBF)的变化。结果表明 :烧伤后大鼠肠上皮细胞线粒体ST3、RCR、P O及肠组织Oext和IMBF均显著降低。与伤前相比 ,最大降幅度分别为 4 2 0 6 %、4 2 15 %、30 94 %、5 9 4 6 %和 5 1 73% (P <0 0 1) ,而ST4 明显高于伤前。相关分析显示 ,IMBF同RCR、Oext和P O呈显著正相关 (r1 =0 92 ,P <0 0 1;r2 =0 96 ,P <0 0 1;r3=0 91,P <0 0 1)。本实验显示 :严重烧伤后肠上皮细胞线粒体呼吸功能受损 ,氧化磷酸化失耦联 ,肠道氧摄取率降低 。
To identify the functional change of intestinal epithelium mitochondria after a burn injury . Wistar rats inflicted with 30% TBSA full thickness burns were used as a model and were randomLy divided into five groups, i.e. normal control(PBD0), postburn groups. State 3 respiration (ST3), state 4 respiration (ST4), respiratory control rate (RCR). Oxygen extraction(Oext), P/O ratio and intestine mucosal blood flow(IMBF) were measured. Results showsed that after burn injury, ST 3, RCR, Oext, P/O ratio and IMBF significantly decreased while ST 4 markedly increased. Correlation analyses showed that IMBF was positively correlated with RCR, Oext and P/O ratio(r 1=0.92, P <0.01;r 2=0.96, P <0.01;r 3=0.91, P <0.01). After burn injury, IMBF and Oxet declined with mitochondria respiration dysfunction and oxidative phosphorylation discoupling.
出处
《基础医学与临床》
CSCD
北大核心
2003年第2期154-156,共3页
Basic and Clinical Medicine
基金
国家重点基础研究发展规划项目 (G19990 5 4 2 0 2 )
