摘要
为探讨肝细胞癌 (HCC)和肝硬化组织中增殖细胞核抗原 (PCNA)及 Ki- 67抗原的表达及意义 ,采用免疫组织化学技术检测 PCNA和 Ki- 67抗原在 HCC和肝硬化组织中的标记指数 (L I)。结果显示 , 、 、 级 HCC的 PCNA L I分别为 (2 6.9± 17.4) %、 (3 3 .1± 2 2 .7) %、 (73 .8± 16.3 ) % ,各级之间差异均有显著性(P均 <0 .0 5 ) ;Ki- 67L I分别为 (2 5 .8± 15 .6) %、 (5 8.2± 18.6) %、 (75 .3± 2 0 .2 ) % ,各级之间差异均有显著性 (P均 <0 .0 5 ) ;但各级 HCC中的 PCNA L I和 Ki- 67L I差异均无显著性 (P均 >0 .0 5 )。肝硬化组织中 PCNAL I为 (8.8± 5 .2 ) % ,Ki- 67L I为 (7.9± 4.4) % ,两者之间差异无显著性 (P>0 .0 5 )。提示 HCC及肝硬化组织中 PCNA和 Ki- 67抗原的阳性率基本一致 ,HCC的分化程度与 PCNA或 Ki- 67密切相关 ;原位检测 HCC组织中PCNA或 Ki- 67抗原的表达 ,有助于判断
To investigate the expression of proliferating cell nuclear antigen (PCNA) and Ki-67 antigen in hepatocellular carcinoma(HCC) and cirrhosis.The immunohistochemical method was used to determine the labeling indices(LI) of PCNA and Ki-67 in 33 cases with cirrhosis and 37 with HCC.Result showed that PCNA and Ki-67 were mainly located in the cell nuclear of HCC.In Ⅰ,Ⅱ and Ⅲ degree HCC,LI of PCNA were(26.9±17.4)%, (33.1±22.7)% and (73.8±16.3)% respectively.The LI of Ki-67 were (25.8±15.6)%, (58.2±18.6)% and (75.3±20.2)% respectively. Significant difference in the LI of PCNA and Ki-67 were found among the various degree HCC(P<0.05).The LI of PCNA and Ki-67 in cirrhosis were(8.8±5.2)% and (7.9±4.4)% respectively,no significant difference between them(P>0.01).These suggest that the positive expression rates of PCNA approximates to the Ki-67 in HCC. The differentiation of HCC is closed related with the LI of PCNA and Ki-67.The determination of PCNA and Ki-67 expression in HCC tissue is an objective and simple method for the prediction of HCC differentiation.
出处
《山东医药》
CAS
北大核心
2003年第12期3-4,共2页
Shandong Medical Journal
基金
上海市科技发展基金资助项目 (No.9841190 0 1)
