摘要
目的观察肝癌癌变过程中端粒酶逆转录酶(h-TERT)、c-myc、增殖细胞核抗原(PCNA)和凋亡活性的改变,分析它们在肝癌发生发展过程中的意义。方法所用标本包括47例原发性肝细胞肝癌、52例肝炎性肝硬变及56例慢性病毒性肝炎,采用原位杂交法检测h-TERT和c-mycmRNA表达,免疫组化SP法检测PCNA表达,用3'-OH末端DNA原位标记技术进行凋亡细胞检测。结果在慢性病毒性肝炎、肝炎性肝硬变及原发性肝细胞肝癌中:h-TERT阳性表达率分别为19.6%(11/56)、82.7%(43/52)和93.6%(44/47);c-myc阳性表达率分别为12.5%(7/56)、40.4%(21/52)和55.3(26/47);凋亡细胞阳性率分别为(27.3±4.7)%、(16.5±2.6)%和(8.7±1.3)%;PCNA分别为(l7.1±2.9)%、(49.3±7.8)%和(62.5±9.1)%。h-TERT、c-myc、PCNA及凋亡细胞阳性表达率在慢性病毒性肝炎、肝炎性肝硬变及原发性肝细胞癌各组中未见相关(P>0.05)。结论肝炎及肝硬变的癌变是一个由量变到质变的过程,h-TERT和c-myc基因的过表达、PCNA升高及凋亡活性下降与肝细胞的恶性转化有关。
Objective To investigate the expressions of human telomerase reverse transcriptase （h-TERT）, c-myc, and proliferating cell nuclear antigen （PCNA） in chronic viral hepatitis （CVH）, liver cirrhosis and primary hepatocellular carcinoma （HCC） and understand their possible role in liver carcinogenesis. Methods Totally 157 liver disease specimens were collected, including 56 CVH, 52 liver cirrhosis and 49 primary HCC specimens. In situ hybridization was performed on these specimens to examine the expressions ofh-TRET and c-myc mRNA, and immunohistochemistry carried out for PCNA detection, with the cell apoptosis detected with in situ ending labeling. Results In the CVH, liver cirrhosis and primary HCC specimens, h-TERT expression was detected at the frequencies of 11/56 （19.6%）, 43/52 （82.7%） and 44/47 （93.6%）, c-myc expression at 7/56 （12.5%）, 21/52 （40.4%） and 26/47 （55.3%）, with apoptotic index of （27.3±4.7）%, （16.5±2.6）% and （8.7±1.3）% and PCNA expression rate of （17.1±2.9）%, （49.3±7.8）% and （62.5 ±9.1）%, respectively. Correlations among h-TERT, c-myc, and PCNA expressions and the apoptotic index were not found in the examined tissues （P〉0.05）. Conclusion Liver carcinogenesis may involve increased h-TERT, c-myc, and PCNA expressions and suppressed cell apoptosis.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2006年第6期821-823,共3页
Journal of Southern Medical University
关键词
肝肿瘤
癌前病变
端粒
凋亡
增殖细胞核抗原
基因表达
liver neoplasms
precancerous conditions
telomerase
apoptosis
proliferating cell nuclear antigen
gene expression
