摘要
目的探讨儿童非霍奇金淋巴瘤 (NHL)与p53蛋白表达的关系。方法用免疫组化En vision法检测 64例 (惰性淋巴瘤 8例 ,侵袭性淋巴瘤 42例 ,高度侵袭性淋巴瘤 1 4例 )NHLp53蛋白 ,根据p53蛋白阳性细胞百分率将表达水平分为 4级 :0级 (阴性 ) ,1级 (1 %～ 2 5 % ) ,2级 (2 6 %～ 50 % ) ,3级 (>50 % )。结果惰性淋巴瘤组 7 8(87 5 % )p53表达为 0级 ,侵袭性淋巴瘤组 36 42 (86 1 % )表达为1级 ,高度侵袭性淋巴瘤组 1 1 1 4 (78 6 % )表达为 2～ 3级 ,儿童NHL蛋白表达水平与其侵袭性密切相关 (P <0 0 1 )。 2 5例随访 7～ 68个月 ,p53 0～ 1级NHL完全缓解率 (CRR ,1 1 1 4 )高于p53 2～ 3级NHLCRR(1 1 1 ,P <0 0 1 ) ,前者生存率 (1 3 1 4 )高于后者 (3 1 1 ,P <0 0 1 )。结论p53蛋白表达阳性细胞百分率是判断NHL恶性度、疗效及预后较可靠的参数。
Objective To study the relationship between childhood non-Hodgkin's lymphoma (NHL) and p53 expression. Methods Sixty-four cases of childhood NHL (indolent lymphoma 8 cases, aggressive lymphoma 42 cases, highly aggressive lymphoma 14 cases) were examined with immunohistochemical staining for p53 oncopotein. Expression levels were divided into 4 degrees with the percentage of positive cells for p53 degree 0 (negative), degree Ⅰ (1%-25%), degree Ⅱ(26%-50%), degree Ⅲ(>50%). Results Expression levels of p53 were strongly associated with the grade of malignancy ( P <0.01). 25 cases were followed up for 7-68 months that the complete remission rate (11/14) in degree 0 and I childhood NHL was higher than that (1/11) in degrees Ⅱ and Ⅲ ( P <0.01). Conclusion The percentage of positive cells for p53 in childhood NHL is a reliable parameter for predicting the grade of malignancy, the curative effect and the prognosis of patients with childhood NHL. It plays an important role in the diagnosis of intermediate-high grade childhood NHL.
出处
《贵州医药》
CAS
2003年第5期409-411,共3页
Guizhou Medical Journal
