摘要
目的探讨非霍奇金淋巴瘤（Non—Hodgkin＇s Lymphorna，NHL）中p53基因第5～8外显子突变情况。方法采用PCR—SSCP法对47例NHL进行p53基因第5～8外显子的点突变研究。结果p53基因第5～8外显子总突变率为48．94％（23／47）。其中第6外显子的突变率最高为17．02％，其次为第8外显子，突交率为12．87％；高度恶性组和低度恶性组突变率分别为50．00％（12／24）、34．78％（8／23）（P＞0．05）；T细胞和B细胞突变率分别为39．29％（11/28）、47．37％（9／19）（P＞0．05）。结论NHL中存在较高p53基因突变率，提示其突变可能参与了NHL的发生发展。高度恶性组p53基因突变倾向于高发。
Objective To investigate the role of p53 gene mutation （exons 5-8）in Non- Hodgkin＇ s Lymphoma（NHL）. Methods A PCR - SSCP method was used to detect the frequence of p53 point mutation in exons 5 to 8 in 47 patients with NHL Results Mutation rate of p53 exons 5-8 was 48.94% （23/47）. Exon 6 was found with the highest mutation rate of 17.02 %, exon 8 with the second rate of 12.87 %. The mutation rate in high grade group and low grade group was 50.00 % （12/24） and 34.78 % （8/23）, respectively （ P 〉0.05）; the mutation rate in T - cell and B- cell derived cases was 39.29% （11/28） and 47.37% （9/19）, respectively （ P〉0.05）. Conclusion play a role in High mutation rate of p53 gene is present in NHL, which indicates that p53 gene mutation might pathogenesis and progression of NHL.
出处
《右江民族医学院学报》
2006年第5期712-714,共3页
Journal of Youjiang Medical University for Nationalities
基金
国家自然科学基金资助项目(30160030)
